BIOTECHNOLOGY - V. H. Herasymenko - 2006

Introduction

Part I. General Biotechnology

Chapter 1. BIOTECHNOLOGY AS A SCIENTIFIC DISCIPLINE

1.1. SUBJECT MATTER AND HISTORY OF BIOTECHNOLOGY

1.2. BIOLOGICAL AGENTS AND METHODS IN BIOTECHNOLOGY

1.3. GOALS AND OBJECTIVES OF BIOTECHNOLOGY

Chapter 2. INTERNATIONAL GLP AND GMP SYSTEMS FOR BIOTECHNOLOGY PRODUCT QUALITY

2.1. THE GLP SYSTEM

2.2 THE GMP SYSTEM

Review Questions

Chapter 3. FUNDAMENTALS OF MOLECULAR BIOLOGY

3.1. NUCLEIC ACIDS

  3.1.1. Chemical composition of nucleic acids

  3.1.2. Structure of nucleic acids

3.2. PROTEIN BIOSYNTHESIS AND ITS REGULATION

  3.2.1. The genetic code

  3.2.2. Stages of protein biosynthesis

  3.2.3. Regulation of protein synthesis

Review Questions

CHAPTER 4. CELL ENGINEERING

4.1. EUKARYOTIC CELL CULTURE

4.2. SOMATIC CELL HYBRIDIZATION TECHNOLOGIES

4.3. NUCLEAR TRANSFER TECHNOLOGY

4.4. GENE TRANSFER TECHNOLOGY INTO SOMATIC CELLS VIA METAPHASE CHROMOSOMES

4.5. BIOTECHNOLOGY OF GENE TRANSFER INTO EUKARYOTIC CELLS VIA DNA (DNA TECHNOLOGY)

4.6. GENE INTRODUCTION. BIOTECHNOLOGY OF TRANSFORMING GERMINAL EMBRYONIC CELLS WITH FOREIGN GENES

Review Questions

CHAPTER 5. FUNDAMENTALS OF GENETIC ENGINEERING

5.1. RECOMBINANT DNA CONSTRUCTION BIOTECHNOLOGY

  5.1.1. Obtaining DNA Fragments

  5.1.2. Plasmids and Viruses as Donor Vectors of Genetic Information

  5.1.3. Construction of Recombinant DNA

  5.1.4. Cloning of Recombinant DNA Molecules

  5.1.5. Expression of Eukaryotic Genes in Prokaryotic Cells

  5.1.6. Prospects and Challenges of Gene Cloning Biotechnology

Review Questions

CHAPTER 6. DNA TECHNOLOGIES

Review Questions

Part II. Special Biotechnologies

Chapter 7. BIOTECHNOLOGY OF PRODUCTION AND APPLICATION OF IMMOBILIZED PREPARATIONS

7.1. ENZYME ENGINEERING. OBJECTIVES OF ENZYME ENGINEERING

7.2. IMMOBILIZATION OF BIOLOGICALLY ACTIVE SUBSTANCES AND CELLS

7.3. ENZYME IMMOBILIZATION. OBJECTIVES OF IMMOBILIZATION

7.4. SUPPORTS FOR ENZYME IMMOBILIZATION

  7.4.1. Organic Polymeric Supports

  7.4.2. Inorganic Supports

  7.4.3. Support Capacity

  7.4.4. Support Modification (Lat. modificatio — change)

  7.4.5. Requirements for Supports

 7.5. METHODS OF ENZYME IMMOBILIZATION

  7.5.1. Physical methods of immobilization

   7.5.1.1. Enzyme immobilization by adsorption on insoluble supports

   7.5.1.2. Methods of mechanical entrapment of enzyme molecules within the support structure

  7.5.2. Chemical methods of immobilization

   7.5.2.1. Basic principles for the design of covalently immobilized enzyme preparations

   7.5.2.2. Characteristics of reagents

7.6. PHYSICOCHEMICAL CHARACTERISTICS OF THE IMMOBILIZED ENZYME

7.7. CLASSIFICATION OF IMMOBILIZED ENZYMES

7.8. CELL IMMOBILIZATION (ADHESION)

  7.8.1. Main methods of cell immobilization

Review questions

CHAPTER 8. APPLICATION OF IMMOBILIZED PREPARATIONS FOR THERAPEUTIC PURPOSES

8.1. IMMOBILIZATION OF PREPARATIONS. SUPPORTS FOR IMMOBILIZATION

8.2. METHODS OF IMMOBILIZATION AND APPLICATION OF PREPARATIONS

8.3. IMMOBILIZED ENZYME THERAPY

Review questions

CHAPTER 9. USE OF IMMOBILIZED ENZYMES IN ANALYTICAL PRACTICE

9.1. ANALYTICAL FLOW REACTORS WITH IMMOBILIZED ENZYMES

9.2. ENZYME MICROCALORIMETRIC SENSORS

9.3. ENZYME ELECTRODES

9.4. BIOLUMINESCENT MICROANALYSIS

9.5. BIOSENSORS WITH IMMOBILIZED ENZYMES

9.6. ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) AND ITS APPLICATIONS

Review questions

CHAPTER 10. APPLICATIONS OF IMMOBILIZED ENZYMES IN BIOTECHNOLOGY

10.1. BIOTECHNOLOGY OF STARCH CONVERSION TO GLUCOSE

  Review Questions

10.2. BIOTECHNOLOGY FOR THE PRODUCTION OF HIGH-FRUCTOSE SYRUPS

  Review Questions

10.3. BIOTECHNOLOGY FOR THE PRODUCTION OF GLUCOSE AND ETHANOL FROM CELLULOSE

  Review Questions

10.4. BIOTECHNOLOGY FOR THE PRODUCTION OF L-MALIC ACID

  Review Questions

10.5. APPLICATION OF IMMOBILIZED ENZYME BIOTECHNOLOGIES IN THE DAIRY INDUSTRY

  Review Questions

10.6. BIOTECHNOLOGY FOR D-PHENYLGLYCINE PRODUCTION

Review Questions

CHAPTER 11. ANTIBIOTIC PRODUCTION BIOTECHNOLOGY

11.1. PRODUCTION OF β-LACTAM ANTIBIOTICS

11.2. MODIFICATION OF β-LACTAM ANTIBIOTICS

  11.2.1. Production of 6-aminopenicillanic acid (6-APA)

  11.2.2. Production of 7-aminocephalosporanic acid - 7-ACA

11.3. DEVELOPMENT OF NEW BIOTECHNOLOGIES FOR THE PRODUCTION AND APPLICATION OF ANTIBIOTICS

Review Questions

Chapter 12. HORMONE PRODUCTION BIOTECHNOLOGY

12.1. PATHWAYS FOR HORMONE PRODUCTION

12.2. PRODUCTION OF INSULIN

  12.2.1. Traditional pathways for insulin production

  12.2.2. Novel technologies for insulin production

12.3. PRODUCTION OF SOMATOTROPIN

  12.3.1. Application of genetically engineered somatotropin in animal husbandry

Review Questions

Chapter 13. BIOTECHNOLOGY OF INTERFERON PRODUCTION

13.1. CLASSES AND TYPES OF INTERFERONS

13.2. TRADITIONAL METHODS FOR INTERFERON PRODUCTION

13.3. GENETIC ENGINEERING METHOD FOR INTERFERON PRODUCTION

13.4. PRODUCTION OF ADVANCED INTERFERONS

13.5. USE OF EXOGENOUS INTERFERON IN VETERINARY MEDICINE AND ANIMAL HUSBANDRY

Review Questions

Chapter 14. BIOTECHNOLOGY FOR THE PRODUCTION OF MONOCLONAL ANTIBODIES (SINGLE-EPITOPE ANTIBODIES)

14.1. TRADITIONAL METHOD FOR ANTIBODY PRODUCTION

14.2. MONOCLONAL ANTIBODIES AND HYBRIDOMA TECHNOLOGY

14.3. APPLICATIONS OF MONOCLONAL ANTIBODIES

Review Questions

Chapter 15. BIOTECHNOLOGY AND VACCINES OF THE FUTURE

Review Questions

Chapter 16. DNA VACCINES

16.1. STRUCTURE

16.2. SELECTION OF GENES

16.3. METHODS AND ROUTES OF ADMINISTRATION

16.4. MODULATION OF THE IMMUNE RESPONSE

16.5. ENHANCING THE IMMUNOGENICITY OF DNA VACCINES

Review Questions

Chapter 17. BIOTECHNOLOGY OF VITAMIN PRODUCTION

Review Questions

Chapter 18. BIOTECHNOLOGY FOR THE PRODUCTION OF BIOLOGICALLY ACTIVE PRODUCTS BASED ON METAL-COMPLEX COMPOUNDS

Review Questions

Chapter 19. BIOTECHNOLOGIES FOR L-AMINO ACID PRODUCTION

19.1. METHODS FOR L-AMINO ACID PRODUCTION

19.2. BIOTECHNOLOGY FOR L-METHIONINE PRODUCTION

19.3. BIOTECHNOLOGY FOR L-TRYPTOPHAN PRODUCTION

19.4. BIOTECHNOLOGY FOR L-LYSINE PRODUCTION

19.5. BIOTECHNOLOGY FOR L-THREONINE PRODUCTION

19.6. BIOTECHNOLOGY FOR L-ASPARTIC ACID PRODUCTION

19.7. BIOTECHNOLOGY FOR L-GLUTAMIC ACID PRODUCTION

Review Questions

Chapter 20. ENZYME PRODUCTION BIOTECHNOLOGY

20.1. SOURCES OF ENZYMES

20.2. CULTIVATION METHODS FOR ENZYME-PRODUCING MICROORGANISMS

20.3. PRODUCTION OF COMMERCIAL FORMS OF ENZYME PREPARATIONS

  20.3.1. Enzyme isolation

  20.3.2. Purification of enzyme preparations

  20.3.3. Enzyme concentration

  20.3.4. Standardization of enzyme preparations

  20.3.5. Identification and indexing of enzyme preparations

20.4. INDUSTRIAL ENZYME PREPARATIONS

Review Questions

Chapter 21. PROTEIN PRODUCTION BIOTECHNOLOGY

21.1. PRODUCTION OF SINGLE-CELL PROTEINS

21.2. MICROORGANISMS AS PROTEIN PRODUCERS

21.3. BASIC FLOWCHART FOR MICROBIAL PROTEIN PRODUCTION

21.4. MICROBIAL PROTEIN PRODUCTION FROM PETROLEUM REFINING BY-PRODUCTS

21.5. MICROBIAL PROTEIN PRODUCTION USING NATURAL GAS (METHANE)

21.6. MICROBIAL PROTEIN PRODUCTION FROM LOWER ALCOHOLS – METHANOL AND ETHANOL

21.7. MICROBIAL PROTEIN PRODUCTION FROM PLANT WASTE HYDROLYSATES

21.8. SINGLE-CELL ALGAL PROTEIN PRODUCTION

21.9. PRODUCTION OF HIGH-PROTEIN FEED SUPPLEMENTS FROM RENEWABLE RAW MATERIALS

  21.9.1. Submerged culture fermentation, or deep cultivation of microorganisms

  21.9.2. Solid-state fermentation of plant raw materials

21.10. MICROBIAL PROTEIN IN HUMAN NUTRITION

Review Questions

Chapter 22. BIOTECHNOLOGIES FOR WASTE MANAGEMENT AND BIOCONVERSION IN THE AGRO-INDUSTRIAL COMPLEX

22.1. ENVIRONMENTAL IMPACT OF LIVESTOCK WASTE

22.2. METHODS OF MANURE MANAGEMENT AND DISPOSAL

  22.2.1. Traditional methods. Use of manure as organic fertilizer

  22.2.2. Mineralization of organic matter in soil and water bodies

  22.2.3. Incorporation of manure into farm animal diets

22.3. NON-TRADITIONAL METHODS. BIOTECHNOLOGY OF BIOGAS PRODUCTION VIA ANAEROBIC WASTE DIGESTION

  22.3.1. Biomethanogenesis and its stages

  22.3.2. Factors affecting biomethanogenesis and their optimization

22.3.3. Technical and technological aspects of biogas production

   22.3.3.1. Composition and global distribution of biogas plants

   22.3.3.2. Design features of biogas plant reactors

   22.3.3.3. Classification of biogas plants by operational principle

   22.3.3.4. Technical and Technological Levels of ADP

  22.3.4. Fractions Generated during Biomethanogenesis

   22.3.4.1. Biogas: Composition and Utilization

   22.3.4.2. Sludge: Composition and Utilization

   22.3.4.3. Liquid Fraction: Composition and Utilization

  22.3.5. Ways to Improve Biogas Production

  22.3.6. Current State of Biogas Production in Europe and Worldwide

  22.3.7. State of Biogas Production in Ukraine

Review Questions

Chapter 23. BIOTECHNOLOGY OF ORGANIC WASTE UTILIZATION VIA VERMICULTIVATION

23.1. GENERAL OVERVIEW AND BIOLOGICAL CHARACTERISTICS OF EARTHWORMS

23.2. METHODS OF EARTHWORM CULTIVATION

23.3. PREPARATION OF SUBSTRATE (FEED) FOR EARTHWORMS

23.4. METHODOLOGY FOR BEDDING SETUP AND TECHNIQUE FOR INTRODUCING THE BREEDING STOCK INTO THE SUBSTRATE

23.5. CONDITIONS FOR MAINTAINING EARTHWORMS IN BEDS

23.6. ASSESSMENT OF THE EARTHWORM POPULATION STATE

23.7. METHODOLOGY FOR BED DIVISION

23.8. WINTER VERMICULTIVATION TECHNOLOGY

23.9. VERMICULTIVATION IN HOME GARDENS

23.10. VERMICULTURE: COMPOSITION AND UTILIZATION

23.11. VERMICOMPOST: COMPOSITION AND UTILIZATION

Review Questions

Chapter 24. BIOTECHNOLOGY FOR PRODUCING BIOMASS FROM THE UNICELLULAR ALGA SPIRULINA

24.1. GENERAL CHARACTERISTICS OF SPIRULINA

24.2. COMPOSITION OF THE NUTRIENT MEDIUM FOR CULTIVATING SPIRULINA

24.3. CHEMICAL COMPOSITION AND NUTRITIONAL VALUE OF SPIRULINA BIOMASS

24.4. APPLICATIONS OF SPIRULINA BIOMASS

24.5. TECHNOLOGY OF SPIRULINA CULTIVATION FOR ANIMAL FEEDING

24.6. CULTIVATION OF SPIRULINA FOR THE PHARMACEUTICAL INDUSTRY

Review Questions

CORE REFERENCES

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