BIOTECHNOLOGY - V. H. Herasymenko - 2006

Part II. Special Biotechnologies

Chapter 7. BIOTECHNOLOGY FOR THE PRODUCTION AND APPLICATION OF IMMOBILIZED PREPARATIONS

7.5. METHODS OF ENZYME IMMOBILIZATION

7.5.2. Chemical methods of immobilization

Chemical immobilization Methods are based on the covalent bonding of enzyme molecules to Supports of either organic or mineral origin. The primary advantage of immobilized enzyme preparations obtained via chemical methods is The formation of strong chemical bonds between the enzyme and the support. This is a crucial operational characteristic of the immobilized biocatalyst, as it prevents the enzyme from washing off the support (desorption) during use. Consequently, this ensures the purity of reaction products—which is vitally important for medical and food-grade manufacturing—as well as stable, reproducible results in analytical systems. Furthermore, chemical immobilization significantly enhances key practical properties, such as substrate Specificity, catalytic activity, and stability. In particular, it is through chemical methods involving multi-point covalent attachment of the Cell/13.html">Protein Structure that the highest degree of enzyme stabilization can be achieved.