BIOTECHNOLOGY - V. H. Herasymenko - 2006

Part II. Special Biotechnologies

Chapter 20. BIOTECHNOLOGY OF ENZYME PRODUCTION

Review Questions

1. In which sectors of the national economy are enzyme preparations used?

2. What SOURCES OF Enzymes exist, and which of them hold promise for large-scale production?

3. What are the advantages of microorganisms as sources of enzymes?

4. What elements are essential for the growth of enzyme-producing microorganisms?

5. What approaches can be used to increase the productivity of producer strains?

6. How do extracellular enzymes differ from intracellular ones, and induced enzymes from constitutive ones?

7. What Methods are used to cultivate enzyme-producing microorganisms?

8. What are the Advantages and disadvantages of the submerged cultivation method for producers?

9. What are the advantages and disadvantages of the surface cultivation method for enzyme producers?

10. What are the Main stages in obtaining commercial forms of enzyme preparations?

11. What methods are used to isolate extracellular enzymes?

12. How are intracellular enzymes isolated, and what methods are applied for Cell disintegration (lysis)?

13. In which industries are crude enzyme preparations utilized?

14. What methods can be employed to obtain purified enzyme preparations?

15. What methods are used for the concentration of enzyme preparations?

16. What is enzyme standardization?

17. How are enzyme preparations identified?

18. How do technical-grade enzyme preparations differ from purified ones, and what are their Applications?

19. How are INDUSTRIAL ENZYME PREPARATIONS classified, and where are they applied?



Last update: 11/08/2026

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