BIOTECHNOLOGY - V. H. Herasymenko - 2006

Part II. Special Biotechnologies

CHAPTER 9. APPLICATION OF IMMOBILIZED ENZYMES IN ANALYTICAL PRACTICE

Review Questions

1. What are the prerequisites for using Enzymes in analytical practice?

2. What is the key characteristic of modern approaches to developing enzyme-based biochemical analysis Methods?

3. What are the advantages of immobilized enzymes over soluble ones in Enzymatic Analysis methods?

4. What types of Supports are used for Enzyme Immobilization in subsequent analytical Applications, and what factors determine the choice of such a support?

5. What circumstances prompted the USE OF IMMOBILIZED enzymes in chemical analysis?

6. What do ANALYTICAL FLOW REACTORS look like, and where are they applied?

7. What supports are used in analytical flow reactors, and what requirements must they meet?

8. What are ENZYME MICROCALORIMETRIC SENSORS, and what are they designed for?

9. What is the main advantage of enzyme microcalorimetric sensors?

10. What are enzyme electrodes, and how are they constructed?

11. Where are enzyme electrodes used?

12. What is the principle underlying the bioluminescent microanalysis method?

13. What supports are used for the immobilization of enzymes and multienzyme systems?

14. In which fields are biosensors with immobilized enzymes applied?

15. What are the Fundamentals of Enzyme-linked immunosorbent assay (ELISA) methods?

16. What markers (labels) are used in ELISA?

17. What are the drawbacks of radioactive labels?

18. What properties should enzyme markers possess?

19. Which substrate labels are most commonly used?

20. What methods are used to introduce the enzyme label (or obtain enzyme conjugates)?

21. How is the Separation of free and bound markers carried out?

22. What is the Classification principle of ELISA methods?

23. What are the KEY FEATURES OF solid-phase methods?

24. What is The Essence of homogeneous ELISA methods and what types of compounds are they used to determine?

25. What are the characteristics of homogeneous Bioluminescence-based assays (LIA)?

26. Where are ELISA methods applied?



Last update: 11/08/2026

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