Fundamentals of Biochemical Engineering, Part 1 - Bailey, J., Ollis, D. 1989



Preface

Introduction to Microbiology

Biophysics and Cell Theory

Cell Structure

Prokaryotic Cells

Eukaryotic Cells

Cell Fractionation

Major Cell Types

Bacteria

Yeasts

Molds

Algae and Protozoa

Plant and Animal Cells

Prospects for Further Study

Chemical Foundations of Life

Lipids

Fatty Acids and Related Lipids

Fat-Soluble Vitamins, Steroids, and Other Lipids

The Chemical Basis of Life

Sugars and Polysaccharides

Chemical Foundations of Life

D-Glucose and Other Monosaccharides

The Chemical Foundations of Life

Disaccharides and Polysaccharides

Chemical Basis of Life

Cellulose

The Chemical Basis of Life

From Nucleotides to RNA and DNA

Structural components of nucleic acids; energy carriers and coenzymes

The Chemical Foundations of Life

Storage of Biological Information, DNA and RNA

Amino Acids and Proteins

Chemical Foundations of Life

Proteinogenic Amino Acids and Polypeptides

Protein Structure

Primary Structure

The Chemical Basis of Life

Three-dimensional structure of proteins; secondary and tertiary structures

Quaternary Structure and Regulation of Biological Processes

Biochemical Compounds of Mixed Structure

Chemical Foundations of Life

Cell walls; peptidoglycans and lipopolysaccharides

The Chemical Basis of Life

Mixed-Structure Biochemical Compounds

Antibodies and Other Glycoproteins

The Hierarchy of Cell Structure

Kinetics of enzyme-catalyzed reactions

Kinetics of Enzyme-Catalyzed Reactions

Enzyme-Substrate Complexes and the Mechanism of Enzyme Action

Kinetics of Simple Single- and Two-Substrate Enzymatic Reactions

Michaelis-Menten Equation

Kinetics of Simple Single- and Two-Substrate Enzyme Reactions

Determination of Parameters in the Michaelis-Menten Equation

Kinetics of simple single- and two-substrate enzymatic reactions

Kinetics of reversible reactions, two-substrate reactions, and enzyme activation by cofactors

Determination of Rate Constants for the Elementary Steps of an Enzymatic Reaction

Determination of Rate Constants for Elementary Steps of Enzymatic Reactions

Relaxation Methods for Studying Kinetics

Determination of Rate Constants for Elementary Steps of an Enzymatic Reaction

Some Results of Transient-State Kinetics Studies

Other types of enzyme reaction rate dependence on substrate concentration

Activation and inhibition of enzymes by substrates

Other Types of Enzymatic Reaction Rate Dependence on Substrate Concentration

Interaction of a Single Enzyme with Several Different Substrates

Regulation of Enzyme Activity

Mechanisms of Reversible Regulation of Enzyme Activity

Analysis of the effect of reversible regulation on the kinetics of enzyme-catalyzed reactions

Other Factors Affecting Enzyme Activity

Other Factors Affecting Enzymatic Activity

Effect of pH on the Kinetics of Enzymatic Reactions in Solutions

Enzyme Reaction Rates and Temperature

Enzyme Inactivation

Mechanisms of Protein Denaturation and Associated Effects

Kinetics of enzyme-catalyzed reactions

Enzyme inactivation

Modeling and kinetics of inactivation processes

Kinetics of Enzyme-Catalyzed Reactions

Enzyme Inactivation

Enzyme Denaturation Induced by Mechanical Factors

Enzyme Stabilization Methods

Enzymatic Reactions in Heterogeneous Systems

Applications of enzyme-catalyzed reactions

Applications of Enzyme-Catalyzed Reactions

Applications of Hydrolytic Enzymes

Hydrolysis of Starch and Cellulose

Proteolytic Enzymes

Applications of enzyme-catalyzed reactions

Applications of hydrolytic enzymes

Applications of esterases

Applications of Enzyme-Catalyzed Reactions

Applications of Hydrolytic Enzymes

Enzyme Mixtures, Pectic Enzymes, and Other Applications of Enzymes

Applications of enzyme-catalyzed reactions

Other applications of enzymatic reactions in solution

Applications of enzymes in medicine

Applications of Enzyme-Catalyzed Reactions

Other Applications of Enzymatic Reactions in Solution

Industrial Applications of Non-Hydrolytic Enzymes and Prospects for Expanding Their Use

Applications of enzyme-catalyzed reactions

Industrial processes involving immobilized enzymes

Applications of Enzyme-Catalyzed Reactions

Technological Processes Involving Immobilized Enzymes

Enzyme Immobilization

Application of enzyme-catalyzed reactions

Technological processes involving immobilized enzymes

Industrial processes

Applications of Enzyme-Catalyzed Reactions

Technological Processes Involving Immobilized Enzymes

Applications of Immobilized Enzymes in Medicine and Chemical Analysis

Application of enzyme-catalyzed reactions

Technological processes involving immobilized enzymes

Cofactor utilization and regeneration

Application of Enzyme-Catalyzed Reactions

Kinetics of Reactions Catalyzed by Immobilized Enzymes

Applications of Enzyme-Catalyzed Reactions

Effect of External Mass Transfer Resistance

Application of enzyme-catalyzed reactions

Kinetics of reactions catalyzed by immobilized enzymes

Modeling of diffusion and reactions within a catalyst particle

Simultaneous mass transfer resistance in the boundary layer and within the catalyst particle

Applications of Enzyme-Catalyzed Reactions

Kinetics of Reactions Catalyzed by Immobilized Enzymes

Effects of Inhibitors, Temperature, and pH on the Catalytic Activity and Inactivation of Immobilized Enzymes

Conclusions

Stoichiometry and Energetics of Metabolic Conversions

Stoichiometry and Energetics of Metabolic Transformations

Principles of Thermodynamics

Interconnectedness of metabolic reactions; ATP and NAD

ATP and other phosphates

Stoichiometry and Energetics of Metabolic Pathways

Oxidation and reduction; coupling with NAD conversion

Stoichiometry and Energetics of Metabolic Transformations

Carbon Catabolism

Embden - Meyerhof - Parnas Pathway

Alternative Pathways of Carbohydrate Catabolism

Stoichiometry and Energetics of Metabolic Conversions

Respiration

Tricarboxylic Acid Cycle

Stoichiometry and Energetics of Metabolic Transformations

Respiratory Chain

Stoichiometry and Energetics of Metabolic Conversions

Photosynthesis and Its Relationship to the Primary Energy Source

Accumulation of Light Energy

Stoichiometry and Energetics of Metabolic Transformations

Photosynthesis and its Relationship to the Primary Energy Source

Electron Transport and Photophosphorylation

Biosynthesis

Biosynthesis of Low-Molecular-Weight Compounds

Stoichiometry and Energetics of Metabolic Conversions

Synthesis of Macromolecular Compounds

Stoichiometry and Energetics of Metabolic Pathways

Transport Across Cell Membranes

Stoichiometry and Energetics of Metabolic Conversions

Passive Diffusion and Coupled Transport

Stoichiometry and Energetics of Metabolic Pathways

Active Transport

Stoichiometry and Energetics of Metabolic Conversions

Organization and Regulation of Metabolism

Stoichiometry and Energetics of Metabolic Transformations

Key Intersection and Branching Points of Metabolic Pathways

Enzyme-Level Regulation of Metabolism

End Products of Metabolism

Products of Anaerobic Metabolism (Fermentation)

Stoichiometry and Energetics of Metabolic Conversions

Partial Oxidation and Its End Products

Stoichiometry and Energetics of Metabolic Transformations

Synthesis of Secondary Metabolites

Stoichiometry of Cell Growth and Product Formation

General Stoichiometry of Cell Growth; Medium Composition and Yield Coefficients

Stoichiometry of Cellular Growth and Product Formation

Elemental Mass Balances and Cellular Growth

Stoichiometry of Cell Growth and Product Formation

Stoichiometry of Metabolite Production Processes

Stoichiometry and Energetics of Metabolic Conversions

Stoichiometry of Energy Metabolism; Estimation of Heat Generation and Associated Yield Coefficients

Stoichiometry of Photosynthesis

Conclusions

Molecular Genetics and Regulatory Systems

Molecular Genetics

Gene Expression Processes

Split genes and mRNA modification in eukaryotes

Post-Translational Modification of Proteins

Induction and Repression; Regulation of Protein Synthesis

DNA Replication and Mutations

General scheme of information flow within the cell

Modification of Cellular DNA Structure

Viruses and Phages; Lysogeny and Transduction

Transformation and Conjugation in Bacteria

Cell Fusion

Molecular genetics and regulatory systems

Industrial applications of research results in microbial genetics and mutant populations

Cellular regulation systems; influence of medium composition

Molecular Genetics and Regulatory Systems

Industrial Applications of Research Results on Microbial Genetics and Mutant Populations

Applications of Auxotrophic Mutants

Industrial Applications of Research on Microbial Genetics and Mutant Populations

Mutants with Modified Regulatory Systems

Recombinant DNA Technology

Enzymes for DNA Cleavage and Ligation

Vectors for Escherichia coli

Identification of Cloned DNA

Expression of Eukaryotic Proteins in E. coli

Molecular genetics and regulatory systems

Recombinant DNA technology

Genetic engineering using other host cells

Molecular Genetics and Regulatory Systems

Recombinant DNA Technology

Conclusion

Growth and Self-Reproduction of an Isolated Cell

Growth and Self-Reproduction of the Isolated Cell

Experimental Methods; Flow Cytometry and Synchronous Cultures

Growth and Self-Reproduction of an Isolated Cell

Cell Cycle of E. coli

The Eukaryotic Cell Cycle

Kinetics of substrate utilization, metabolite production, and biomass formation in cell cultures

Kinetics of substrate utilization, metabolic product formation, and biomass growth in cell cultures

Ideal reactors for studying cell growth kinetics

Ideal batch reactor

Kinetics of substrate utilization, metabolite and biomass production in cell cultures

Continuous stirred-tank reactor (CSTR)

Kinetics of substrate utilization, metabolite production, and biomass formation in cell cultures

Kinetics of balanced growth

Kinetics of substrate utilization, metabolic product formation, and biomass generation in cell cultures

Monod equation for cell growth kinetics

Effects of endogenous metabolism and maintenance metabolism on cell growth kinetics

Kinetics of substrate utilization, metabolic product formation, and biomass accumulation in cell cultures

Other kinetics equations for cell growth

Kinetics of substrate utilization, metabolite production, and biomass formation in cell cultures

Influence of other environmental parameters on cell growth kinetics

Kinetics of substrate utilization, metabolic product formation, and biomass generation in cell cultures

Transient-state cell growth kinetics

Main phases of cell growth in batch reactors

Kinetics of substrate utilization, metabolite production, and biomass formation in cell cultures

Unstructured models of cell growth in batch processes

Growth of filamentous organisms

Structured models of cell growth kinetics

Compartmental models

Kinetics of substrate utilization, metabolic product formation, and biomass accumulation in cell cultures

Metabolic models

Modeling cell growth as an optimal process

Kinetics of substrate utilization, metabolite production, and biomass formation in cell cultures

Kinetics of metabolite production

Unstructured models

Chemically structured models for the kinetics of cellular product formation

Modeling the kinetics of metabolite production based on molecular transformation mechanisms; genetically structured models

Kinetics of metabolic product formation by filamentous organisms

Segregated models for cell growth kinetics and metabolite production

Kinetics of substrate utilization, metabolite and biomass production processes in cell cultures

Kinetics of thermal death of cells and spores

Kinetics of substrate utilization, metabolite production, and biomass formation in cell cultures

Conclusion

Transport Phenomena in Biotechnological Systems

Mass Transfer Between Gas and Liquid Phases in Cellular Systems

Transport Processes in Biotechnological Systems

Basic Principles of Mass Transfer Theory

Transport Phenomena in Biotechnological Systems

Gas-Liquid Mass Transfer in Cellular Systems

Oxygen Uptake Rate in Cellular Metabolism Processes

Transport phenomena in biotechnological systems

Determination of the oxygen transfer rate

Transport Processes in Biotechnological Systems

Determination of Oxygen Transfer Rate

Determination of a$ Using Chemical Reactions between Gases and Liquids

Mass Transfer Involving Freely Rising and Freely Falling Bodies

Transport phenomena in biotechnological systems

Mass transfer involving freely rising and freely falling bodies

Mass transfer coefficients for gas bubbles and bubble streams

Mass transfer involving freely rising and falling bodies

Determination of interfacial area and gas holdup of a dispersed system

Transport Processes in Biotechnological Systems

Mass Transfer by Forced Convection

General Principles and Basic Similarity Criteria

Equations for Determining Mass Transfer Coefficients and Interfacial Area

Transport phenomena in biotechnological systems

Determination of kia' and power consumption of stirred-tank and sparged reactors

Transport Processes in Biotechnological Systems

Mass Transfer Across Free Surfaces

Transport processes in biotechnological systems

Other factors affecting kia'

Determination of diffusion coefficients

Ionic strength

Transport phenomena in biotechnological systems

Surfactants

Non-Newtonian fluids

Mathematical models of non-Newtonian fluids and corresponding parameters

Transport processes in biotechnological systems

Suspensions

Transport phenomena in biotechnological systems

Solutions of macromolecular compounds

Power consumption and mass transfer in biotechnological processes with non-Newtonian fluids

Transport processes in biotechnological systems

Scale-up and mass transfer

Transport phenomena in biotechnological systems

Heat transfer

Heat transfer equations

Sterilization of gases and liquids by filtration