Amino Acids, Peptides and Proteins - Devenyi T., Gergely J. 1976



Preface to the Russian edition

Selected methodological aspects of analytical protein research

Study of native proteins

Electrophoresis of native proteins

Some methodological aspects of analytical protein research

The zone electrophoresis method

Methodological aspects of the analytical study of proteins

Study of native proteins by immunochemical methods

Selected Methodological Issues in the Analytical Study of Proteins

Study of Native Proteins

Ion-Exchange Chromatography of Proteins

Some methodological aspects of analytical protein research

Study of native proteins

Protein fractionation by gel filtration

Some methodological aspects of the analytical study of proteins

Membrane filtration

Some methodological aspects of analytical protein research

Methodological aspects of protein amino acid sequence analysis

Protein Analysis by Low-Voltage Electrophoresis

Low-Voltage Paper Electrophoresis

Low-Voltage Paper Electrophoresis

Protein analysis by low-voltage electrophoresis

Low-voltage paper electrophoresis

Staining procedure

Protein analysis using low-voltage electrophoresis

Quantitative evaluation of protein separation in low-voltage paper electrophoresis

Protein analysis by low-voltage electrophoresis

Common sources of error in low-voltage paper electrophoresis

Protein analysis using low-voltage electrophoresis

Determination of protein content

Protein analysis by low-voltage electrophoresis

Cellulose acetate membrane electrophoresis

Analysis of proteins by low-voltage electrophoresis

Agar gel electrophoresis

Protein analysis using low-voltage electrophoresis

Starch gel electrophoresis

Vertical starch gel electrophoresis

Protein analysis by low-voltage electrophoresis

Polyacrylamide gel electrophoresis

Zone electrophoresis in polyacrylamide gel

Protein analysis using low-voltage electrophoresis

Disc electrophoresis

Methods of medium- and high-voltage electrophoresis

Apparatus for medium-voltage horizontal electrophoresis and its application

Methods of Medium- and High-Voltage Electrophoresis

The Diagonal Electrophoresis Method

Isolation of Cystine-Containing Peptides

Methods of medium- and high-voltage electrophoresis

Diagonal electrophoresis method

Isolation of methionine-containing peptides

Medium- and High-Voltage Electrophoresis Methods

Diagonal Electrophoresis Method

Isolation of Histidine-Containing Peptides

Methods of medium- and high-voltage electrophoresis

Diagonal electrophoresis method

Isolation of lysine-containing peptides

Medium- and high-voltage electrophoresis methods

Isolation of the C-terminal peptide from tryptic protein hydrolysates

Medium- and High-Voltage Electrophoresis Methods

High-Voltage Electrophoresis Method

Methods of immunochemical analysis

Preparation of immune serum

Immunochemical Analysis Methods

Preparation of Immune Serum

Immunization protocols without adjuvant

Methods of Immunochemical Analysis

Immunization Protocols with Adjuvants

Protein Analysis Using Precipitation Reactions

Qualitative Precipitation Reaction

Protein Analysis by Precipitation Reaction

Determination of Precipitating Antibody Titer by Antigen Dilution

Determination of the Equivalence Zone in the Antigen-Antibody Reaction

Protein Analysis via Precipitation Reactions

Ring precipitin test (precipitation reaction at the interface of layers containing antigen and antibodies

Protein Analysis by Precipitation Reaction

Heidelberger's Quantitative Precipitation Reaction

Protein Analysis by Gel Diffusion Methods

Oudin's Method of Simple Linear Gel Diffusion

Protein Analysis by Gel Diffusion

Method of Double (One-Dimensional) Linear Gel Diffusion

Protein Analysis by Gel Diffusion Methods

Ouchterlony Double Gel Diffusion Method

Immunoelectrophoresis

Analysis of Proteins by Gel Diffusion Methods

Comparative Protein Analysis by the Osserman Method

Protein Analysis by Gel Diffusion Methods

Absorption Immunoelectrophoresis

Immunogel Filtration

Electrosyneresis

Qualitative and quantitative analysis of proteins using electrophoresis in antibody-containing agarose gel

Quantitative Immunodiffusion Methods

Analysis of Proteins by Gel Diffusion

Immunodiffusion Analysis of Proteins on Cellulose Acetate Membranes

Hydrolysis of proteins and peptides

Preparation of sample material for hydrolysis

Oxidation of proteins and peptides with performic acid

Hydrolysis of Proteins and Peptides

Preparation of Samples for Hydrolysis

Reduction and Carboxymethylation of Proteins and Peptides

Sample Preparation for Hydrolysis

Hydrolysis of Proteins and Peptides in 6 N HCl

Hydrolysis of proteins and peptides

Partial acid hydrolysis of proteins and peptides

Tryptic hydrolysis of proteins and peptides

Hydrolysis of proteins and peptides by chymotrypsin

Hydrolysis of proteins and peptides by pepsin

Hydrolysis of proteins and peptides by papain

Hydrolysis of Proteins and Peptides

Cyanogen bromide cleavage of proteins

Quantitative determination of amino acids using automatic amino acid analyzers

Paper chromatography of amino acids and peptides

Ion-exchange chromatography of peptides

Fractionation of acidic peptides on an Amberlite IR-4B column

Chromatography of basic peptides on an Amberlite IRC-50 column

Fractionation of peptides on a Dowex 50x2 column in non-volatile buffer solutions

Fractionation of peptides on a Dowex 50x2 column in volatile solvents

Peptide chromatography on a CM-cellulose column

Chromatography of peptides on a DEAE-cellulose column

Protein chromatography

Protein chromatography on a DEAE-cellulose column

Chromatography on a DEAE-cellulose column in the OH- form

Protein Chromatography

Protein Chromatography on a DEAE-Cellulose Column

DEAE-cellulose in the Cl- form

Protein chromatography

Protein chromatography on a DEAE-cellulose column

Chromatography on DEAE-cellulose in the presence of urea

Protein Chromatography

Serum protein chromatography on a DEAE-cellulose column

Protein chromatography on a cation-exchange cellulose column

CM-cellulose

Fractionation of blood serum proteins on a phosphocellulose column

Protein Chromatography on a Cation-Exchange Cellulose Column

Fractionation of Blood Serum Proteins on a CM-Cellulose Column

Fractionation of serum proteins on a DEAE-Sephadex column

Isolation of IgG from blood serum using batch fractionation on DEAE-Sephadex

Gel Filtration of Proteins and Peptides

Isolation of IgG from serum by batch fractionation on DEAE-Sephadex

Desalting of protein solutions on a Sephadex column

Concentration of Protein Solutions Using Sephadex

Fractionation of Serum Proteins by Gel Filtration

Separation of high-molecular-weight fragments by Sephadex gel filtration

Gel filtration of proteins and peptides

Prefractionation of enzymatic hydrolysates on a Sephadex G-50 column

Gel Filtration of Proteins and Peptides

Protein separation by recycling gel filtration

Thin-Layer Chromatography (D. A. Medgyesi)

Thin-Layer Chromatography of Amino Acids and Their Derivatives

DNP-amino acids

Thin-layer chromatography of amino acids and their derivatives

PTH-amino acids

Thin-Layer Chromatography of Amino Acids and Their Derivatives

Dansyl Amino Acids

Peptides

Thin-Layer Gel Filtration

Ion-exchange chromatography in a fixed bed of ion exchanger

Types of "Fixion" plates

Ion-Exchange Chromatography in a Fixed Ion-Exchanger Bed

Application of “Fixion 50x8” Plates

Ion-exchange chromatography on a fixed bed of ion exchanger

Equilibration of "Fixion 50x8" plates

Ion-exchange chromatography in a fixed bed of ion exchanger

Sample application

Preparation of ninhydrin solution

Ion-Exchange Chromatography in a Fixed Ion-Exchanger Bed

Preparation of Collidine-Ninhydrin Reagent

Ion-exchange chromatography in a fixed ion-exchanger bed

Applications

Anion-exchange chromatography on "Fixion" plates

End-group analysis and stepwise degradation of proteins and peptides

Determination of N-terminal groups by the 2,4-dinitrofluorobenzene method

Analysis of Terminal Groups and Stepwise Degradation of Proteins and Peptides

Determination of N-Terminal Groups by the 2,4-Dinitrofluorobenzene Method

Dinitrophenylation of Proteins

End-group analysis and stepwise degradation of proteins and peptides

Determination of N-terminal groups by the 2,4-dinitrofluorobenzene method

Hydrolysis of DNP-protein or DNP-peptide and extraction of DNP-derivatives

End-Group Analysis and Stepwise Degradation of Proteins and Peptides

Determination of N-Terminal Groups by the 2,4-Dinitrofluorobenzene Method

Paper Chromatography of DNP-Amino Acids

End-group analysis and stepwise degradation of proteins and peptides

Determination of N-terminal groups by the 2,4-dinitrofluorobenzene method

Identification of DNP-amino acids by thin-layer chromatography

End-Group Analysis and Stepwise Degradation of Proteins and Peptides

Determination of N-Terminal Groups by the 2,4-Dinitrofluorobenzene Method

Indirect Identification of DNP-Amino Acids

End-group analysis and stepwise degradation of proteins and peptides

Determination of N-terminal groups by the 2,4-dinitrofluorobenzene method

Quantitative determination of DNP derivatives

Preparation of DNP-amino acids

Preparation of ε-DNP-lysine

Determination of N-terminal amino acids by the dansyl method

Terminal group analysis and stepwise degradation of proteins and peptides

Determination of the N-terminal amino acid sequence by reaction with phenyl isothiocyanate

End-group analysis and stepwise degradation of proteins and peptides

Protein degradation using PTC

Amino acid sequence analysis of peptides by the dansyl-Edman method

Determination of amino acid sequences in peptides by Edman degradation and quantitative amino acid analysis

Identification and determination of PTH derivatives

Preparation of PTH-amino acids

N-terminal sequence determination using leucine aminopeptidase

Terminal group analysis and stepwise degradation of proteins and peptides

C-terminal determination by hydrazinolysis

End-group analysis and stepwise degradation of proteins and peptides

Determination of the C-terminal amino acid sequence using carboxypeptidase

Gas chromatographic analysis of amino acid derivatives. State of the art. Capabilities and limitations of the method.

Introduction

Gas chromatographic analysis of amino acid derivatives. Current state. Scope and limitations of the method

Principle of the method

Gas chromatographic analysis of amino acid derivatives. Current status. Capabilities and limitations of the method.

Procedure

Gas chromatographic analysis of amino acid derivatives. Current status. Capabilities and limitations of the method

Special instrument requirements and experimental conditions for GC analysis of amino acid derivatives and peptides

Gas Chromatographic Analysis of Amino Acid Derivatives. State of the Art. Capabilities and Limitations of the Method

Special Instrumental Requirements and Experimental Conditions for GC Analysis of Amino Acid Derivatives and Peptides

Gas Chromatographs for Amino Acid Analysis and Their Requirements

Gas chromatographic analysis of amino acid derivatives. Current status. Capabilities and limitations of the method

Special instrument requirements and experimental conditions for GC analysis of amino acid derivatives and peptides

Quantitative evaluation of gas chromatograms

Gas chromatographic analysis of amino acid derivatives. Current status. Capabilities and limitations of the method.

Properties of amino acid derivatives

Preparation of amino acid derivatives

Gas chromatographic analysis of amino acid derivatives. Current status. Scope and limitations of the method.

Preparation of amino acid derivatives by functional group protection

Gas chromatographic analysis of amino acid derivatives: Current state, capabilities, and limitations of the method

Formation of derivatives during the chemical transformation of amino acids

Gas chromatographic analysis of amino acid derivatives. Current status. Capabilities and limitations of the method.

Formation of derivatives during amino acid degradation

Gas chromatographic analysis of amino acid derivatives. Current status. Scope and limitations of the method

Pyrolysis of amino acids

Gas chromatographic analysis of amino acid derivatives. State of the art. Capabilities and limitations of the method.

Gas chromatography of amino acid derivatives

Gas chromatographic analysis of amino acid derivatives. Current status. Capabilities and limitations of the method

Challenges in quantitative amino acid analysis

Gas chromatographic analysis of amino acid derivatives. Current status. Scope and limitations of the method

Gas chromatography of peptides

Gas chromatographic analysis of amino acid derivatives. Current status. Capabilities and limitations of the method

Biemann et al. method

Gas chromatographic analysis of amino acid derivatives. State of the art. Capabilities and limitations of the method.

Weygand et al. method