Amino Acids, Peptides and Proteins - Dévényi T., Gergely J. 1976

Gel Filtration of Proteins and Peptides
Concentration of Protein Solutions Using Sephadex

Principle of the method. Due to its high hydrophilicity, dry Sephadex added to the solution to be concentrated rapidly absorbs Water, thereby concentrating the high-molecular-weight compounds remaining in the solution.

Applications. Concentration of chromatographic fractions, Proteins isolated by other Fractionation Methods, biological fluids, etc.

PROCEDURE

Dry Sephadex G-25 (coarse) is added to the solution to be concentrated, the suspension is thoroughly mixed and, after 10 min, centrifuged or filtered. Each gram of dry Sephadex G-25 absorbs approximately 2.5 g of water. Following Sephadex Treatment, the resulting filtrate or supernatant becomes more concentrated than the initial solution, while its pH and Ionic strength remain virtually unchanged.

NOTES

1. Performing the described procedure once allows the solution to be concentrated approximately 3-fold. If further concentration is required, the process is repeated with a fresh portion of dry Sephadex.

2. Washed and dried Sephadex can be reused for concentrating solutions.



Last update: 06/08/2026

Editorial and Educational Adaptation: This material has been compiled based on the primary/original source text. The project team performed an editorial review, corrected technical inaccuracies, structured sections, and adapted the content for an educational format.

What was processed:

  • elimination of formatting defects (OCR errors, structural breaks, corrupted characters);
  • editorial organization of content;
  • standardization of terminology in accordance with academic sources;
  • verification of factual statements against the original source text.

All mentions of the author, publication year, and origin of the primary text have been preserved in accordance with the source.