Protein Chemistry - Part 1 - General Protein Chemistry - Ashmarin I. P. 1968
Introduction
Isolation and Purification of Proteins
General recommendations that are useful to follow during the isolation and purification of proteins
Removal of Non-Protein Substances
Extraction of Proteins from Cells and Tissues
Separation of Protein Mixtures
Methods Based on Differences in Protein Solubility
Methods Based on Differences in Protein Molecular Weight and Size
Ion-Exchange Chromatography of Proteins
Assessment of Protein Purification Efficiency
Quantitative Determination of Proteins
Methods for Assessing Protein Purification Efficiency and Homogeneity
Chemical Composition of Proteins
Hydrolysis of Proteins and Polypeptides
Chemical composition of proteins
Determination of amino acids in protein hydrolysates and their fractionation
General characteristics of amino acids
Chemical Composition of Proteins
Chemical Reactions of Amino Acids
Reactions of the Carboxyl Group
Reactions Involving the Simultaneous Participation of $\alpha$-Amino and $\alpha$-Carboxyl Groups
Chemical composition of proteins
Chemical reactions of amino acids
Reactions for individual amino acids
Amino acid composition of proteins
Chemical reactions of proteins, determination of terminal and functional groups in proteins
Chemical reactions of proteins
Reactions for sulfhydryl and disulfide groups
Reactions for free amino groups
Reactions involving carboxyl groups
Reactions involving aliphatic hydroxyl groups
Some other chemical reactions of proteins
Determination of N- and C-terminal amino acids
The method was proposed by Lens in 1949.
Primary structure of the protein molecule
Determination of the number of polypeptide chains. Chain separation
Study of the sequence of amino acid residues in peptide chains
Primary Structure of the Protein Molecule
Determination of the Number and Location of Disulfide Bonds
Spatial Organization of the Protein Molecule
Main Types of Bonds in a Protein Molecule and Levels of Its Organization
Secondary Structure of Polypeptides
Methods for Studying the Secondary Structure of Proteins and Polypeptides
Rotation of the Plane of Light Polarization (Optical Activity)
Spatial organization of the protein molecule
Methods for studying the secondary structure of proteins and polypeptides
Light absorption in the ultraviolet region of the spectrum
Spatial Organization of the Protein Molecule
Methods for Studying the Secondary Structure of Proteins and Polypeptides
Helix-Coil Transition for Polypeptides
Tertiary Structure of Proteins. X-ray Diffraction Analysis of Proteins
Determination of the size and shape of protein molecules
Determination of the Size and Shape of Protein Molecules
Osmotic Pressure of Protein Solutions
Determination of the size and shape of protein molecules
Viscosity of protein solutions
Determination of the Size and Shape of Protein Molecules
Light Scattering by Protein Solutions
Analytical Ultracentrifugation
Determination of the size and shape of protein molecules
Analytical ultracentrifugation
Sedimentation equilibrium method
Comparative characteristics of methods used to estimate the size and shape of protein molecules
Protein Electrochemistry. Protein-Water Interactions
Amino Acids as Dipoles. Ionization of Amino Acids
Protein Electrochemistry. Protein-Water Interaction
Protein Ionization. Binding of Hydrogen Ions
Protein Electrochemistry. Interaction of Proteins with Water
Protein Electrophoresis in Liquid Medium. Moving Boundary Method
Dielectric Constants of Proteins, Relaxation Time, and Protein Molecular Shape
Electrochemistry of Proteins. Protein-Water Interactions
Protein Hydration. Partial Specific Volumes of Proteins in Solution
Protein electrochemistry. Protein-water interactions
Properties of Denatured Proteins