BIOTECHNOLOGY - Inshyna N.M. - 2009
INTRODUCTION
BIOTECHNOLOGY AS A SCIENCE. MODERN ACHIEVEMENTS OF BIOTECHNOLOGY
History of biotechnology development
CHAPTER 1. GENETIC ENGINEERING
Enzymes for nucleic acid restriction and modification
Chemical synthesis of DNA and gene design
Methods for introducing recombinant DNA into cells
Recombinant gene expression systems
Polymerase chain reaction as a method for DNA amplification
New directions in genetic research: genomics, behavioral genetics
Vectors for plant cell transformation
Plant growth-promoting bacteria
Animal genetic engineering. Technology for producing transgenic animals
Pros and cons of using transgenic plants and animals in agriculture
CHAPTER 2. PROTEIN ENGINEERING
Rational design and directed evolution of protein molecules
Mutations and mutagenesis, and their applications in protein engineering
Recent advances in protein engineering. Modification of enzyme properties
Plant cell engineering. History of the plant cell culture method
Main directions of plant cell engineering
Plant protoplasts as objects for biological design
Artificial associations of higher plant cell cultures and microorganisms
Animal cell cultures. History of the method
Stromal and hematopoietic stem cells
CHAPTER 4. MEDICAL BIOTECHNOLOGY
Production of Human Recombinant Proteins
Immunobiotechnology. Technology of Monoclonal Antibody Production
CHAPTER 5. INDUSTRIAL BIOTECHNOLOGY
Stages of Biotechnological Production
Types of Industrial Bioprocesses
Production of Microbial Biomass
Industrial Protein Synthesis Using Recombinant Microorganisms
Production of Microbial Cell Components. Industrial Enzyme Production
Technology for Microbial Lipid Production
Production of Microbial Primary Metabolites
Production of Alcohols, Organic Acids, and Vitamins
CHAPTER 6. ENVIRONMENTAL BIOTECHNOLOGY
Wastewater Treatment Biotechnology
Wastewater Pollution Indicators
The Role of Aquaculture in Wastewater Treatment
Biodegradation and Conversion of Municipal and Industrial Wastes