BIOCHEMISTRY - Laboratory Practical Course - NAU 2015

INTRODUCTION

The main objective of the laboratory practical course in biochemistry is to introduce students to Modern Methods of biochemical research, reinforce their theoretical knowledge, and develop practical skills and competencies based on the latest achievements in biochemical science.

The manual outlines the methodology and Procedures for conducting laboratory work. Through these experiments, students master biochemical Research Methods and acquire The ability to analyze experimental results. A dedicated section covers safety regulations for working in a biochemical laboratory, followed by a list of References used in preparing the manual.

The practical course includes laboratory sessions covering the core sections of biochemistry: CARBOHYDRATES, Lipids, Nucleic Acids, Proteins, Enzymes, and Vitamins. The Structure of the manual complies with the approved working curriculum for the discipline of Biochemistry. The first module, "BIOCHEMICAL COMPONENTS OF The Cell," contains five laboratory exercises focused on detecting protein Amino Acids, carbohydrates, lipids, and vitamins, as well as studying their chemical properties. The second module, "Enzymes and Metabolic Pathways. METABOLISM/26.html">Energy Metabolism," includes four exercises that demonstrate The Role of enzymes in Chemical Reactions characteristic of living organisms and illustrate the main Metabolic pathways of energy metabolism, specifically The Tricarboxylic Acid Cycle and the Respiratory Chain. The fourth module, "Metabolism of Major Classes of Biomolecules," consists of three laboratory sessions highlighting the metabolic Transformations of Amino acids and carbohydrates, alongside The properties of lipids. The fifth module comprises a single laboratory exercise dedicated to the DETECTION OF Hormones in test samples. Each laboratory work outlines the underlying Principle of the method, provides the necessary structural formulas and reactions, lists required Materials, Reagents, and equipment, details the step-by-step procedure, and includes Review Questions. These questions require not only rote memorization but also analytical thinking to help students interpret their findings, which is crucial for preparing them to conduct independent scientific research.