BIOCHEMISTRY - Laboratory Manual - NAU 2015

CONTENTS

Introduction……………………………………………………………………......3

SAFETY GUIDELINES FOR LABORATORY

PRACTICE…………………………….…………………………………………4

MODULE I BIOCHEMICAL COMPONENTS OF The Cell............................5

LABORATORY WORK 1. Qualitative reactions for Amino Acids………..…….5

LABORATORY WORK 2. QUALITATIVE REACTION FOR Peptides and Proteins. Protein fractionation by salting-out. Determination of the main constituent components of Nucleoproteins……………………………......13

LABORATORY WORK 3. Qualitative reactions for Monosaccharides,

 reducing Disaccharides, starch ……………..……….….......….20

LABORATORY WORK 4 DETERMINATION OF CHEMICAL parameters of fats ……26

LABORATORY WORK 5. DETECTION OF Vitamins in model

 solutions ………..…………………………………………………..…….32

MODULE II Enzymes AND METABOLIC PATHWAYS. METABOLISM/26.html">Energy Metabolism……………………………..…....42

LABORATORY WORK 6. Detection of enzyme presence in biological fluid. Determination of specific properties of
enzymes as biological catalysts ………………………..……….42

LABORATORY WORK 7. Investigation of the Specificity of hydrolytic enzymes action ……………………………………….…..........49

LABORATORY WORK 8. Experimental confirmation of The Tricarboxylic Acid Cycle functioning ………………………...53

LABORATORY WORK 9. Experimental confirmation of the Respiratory Chain functioning …..……………………………...56

MODULE IV METABOLISM OF MAJOR CLASSES

 OF Biomolecules ……………………………………………...……..…..65

LABORATORY WORK 10. Experimental confirmation of amino acid metabolic transformations ………………………....…….65

LABORATORY WORK 11. EXPERIMENTAL MODELING OF Glycolysis reactions …………….…………………………………..….…...........72

LABORATORY WORK 12. Evidence of the Structure and properties of Lipids ….75

MODULE V HORMONAL REGULATION OF METABOLISM.……...81

LABORATORY WORK 13. DETECTION OF Hormones in model

 solutions ………………………........................................................…….81

References……………………………………………................90