BIOCHEMISTRY - Textbook - Ostapchenko L. I. - 2012

PREFACE

INTRODUCTION

Chapter 1. STRUCTURE AND REACTIVITY OF BIOORGANIC COMPOUNDS

   1.1. Molecules as components of living organisms

     1.1.1. Enantiomers and chiral compounds

   1.2. Reactions involving bioorganic molecules

   1.3. Selected classes of bioorganic compounds and their biological significance

     1.3.1. Hydrocarbons and hydroxy compounds

     1.3.2. Carbonyl compounds

     1.3.3. Carboxylic acids

     1.3.4. Bioorganic nitrogen compounds

     1.3.5. Heterofunctional compounds

     1.3.6. Heterocyclic compounds

   Review questions

Chapter 2. THE ROLE OF WATER, MACRO- AND MICROELEMENTS IN VITAL ACTIVITY OF ORGANISMS

   2.1. Unique property of water to dissolve substances of diverse origins

   2.2. Bound water

   2.3. Hydrophobic interactions, macromolecules, and water

   2.4. Macro- and microelements

   Review questions

Chapter 3. THE CELL AND EXTRACELLULAR MATRIX

   3.1. Molecular and supramolecular organization of the cell

     3.1.1. Prokaryotic cells

     3.1.2. Eukaryotic cells

   3.2. Cell Classification

   3.3. Extracellular Matrix

   Review Questions

Chapter 4. MOLECULAR ORGANIZATION AND BIOLOGICAL FUNCTIONS OF MEMBRANES

   4.1. Structure of Biological Membranes

   4.2. Molecular Organization of Membranes

     4.2.1. Lipids

     4.2.2. Membrane Proteins

     4.2.3. Carbohydrates, Water, and Inorganic Ions

   4.3. Membrane Functions

     4.3.1. Membrane Transport

     4.3.2. Pores, Channels, and Electrogenesis

     4.3.3. Membrane Receptors and Extracellular Signal Transduction

   4.4. Membrane Fusion in Exocytosis and Endocytosis

   Review Questions

Chapter 5. STRUCTURE, PROPERTIES, AND FUNCTIONS OF PROTEINS

   5.1. Structure and Properties of Proteinogenic Amino Acids. Peptide Bonds

     5.1.1. General Structural Features of Amino Acids

     5.1.2. Classification of Amino Acids

     5.1.3. Chemical Reactions Used for Amino Acid Detection

     5.1.4. The Peptide Bond. Structure and Biological Properties of Peptides

   5.2. Protein Structure

     5.2.1. Primary Protein Structure

     5.2.2. Secondary Protein Structure

     5.2.3. Tertiary Protein Structure

     5.2.4. Supersecondary protein structure

     5.2.5. Quaternary protein structure

   5.3. Formation of the three-dimensional protein structure in the cell

   5.3.1. Protein renaturation

   5.3.2. Structure and functional role of chaperones in protein folding

   5.3.3. Diseases associated with protein folding disorders

   5.4. Protein function

     5.4.1. Active site of proteins and selectivity of ligand binding

     5.4.2. Substances affecting protein function

   5.5. Functional characteristics of oligomeric proteins

     5.5.1. Structure and functions of myoglobin

     6.5.2. Structure and functions of hemoglobin

   5.6. Protein diversity and classification

     5.6.1. Classification of proteins by molecular shape

     5.6.2. Classification of proteins by chemical structure

     5.6.3. Classification of proteins by function

   5.7. Physicochemical properties of proteins and methods for their isolation

     5.7.1. Physicochemical properties of proteins

     5.7.2. Methods for protein isolation and purification

   Review questions

Chapter 6. AMINO ACID METABOLISM AND FUNCTIONS. PROTEIN BIOSYNTHESIS

   6.1. Sources and pathways of amino acid utilization in cells

6.2. Biological value of proteins

     6.2.1. Nitrogen balance

     6.2.2. Nutritional quality of dietary proteins

   6.3. Protein Digestion

     6.3.1. Protein Digestion in the Stomach

     6.3.2. Protein Digestion in the Intestine

     6.3.3. Protection of Cells from Protease Action

     6.3.4. Transport of Amino Acids to Cells

     6.3.5. Disorders of Protein Digestion and Amino Acid Transport

   6.4. Amino Acid Catabolism

     6.4.1. Transamination

     6.4.2. Amino Acid Deamination

   6.5. Ammonia Metabolism. Urea Cycle

     6.5.1. Ammonia Binding (Detoxification)

     6.5.2. Urea Cycle

   6.6. Pathways of Nitrogen-Free Residue Metabolism in Amino Acids

   6.7. Biosynthesis of Non-Essential Amino Acids

   6.8. Metabolism of Individual Amino Acids

     6.8.1. Metabolism of Serine and Glycine

     6.8.2. Metabolism of Methionine and Cysteine

     6.8.3. Metabolism of Phenylalanine and Tyrosine

   6.9. Nitrogen-Containing Compounds: Amino Acid Derivatives

     6.9.1. Decarboxylation of Amino Acids and Their Derivatives

     6.9.2. Nitrogen-Containing Compounds: Histidine Derivatives

     6.9.3. Role of Arginine and Ornithine

   6.10. Protein Biosynthesis

     6.10.1. Main Components of the Translational Apparatus

     6.10.2. Key Properties of the Genetic Code

     6.10.3. Amino acid activation and aminoacyl-tRNA formation

     6.10.4. Ribosomes: structure and functions

     6.10.5. Translation initiation. Features of the initiation process in prokaryotes and eukaryotes

     6.10.6. Polypeptide chain elongation

     6.10.7. Translation termination

     6.10.8. Energy supply for translation. Protein synthesis inhibitors

     6.10.9. Protein folding and processing

     6.10.10. Regulation of protein synthesis

   Review questions

Chapter 7. ENZYMOLOGY

   7.1. General characteristics of enzymes as biological catalysts

     7.1.1. Specificity

     7.1.2. Catalytic efficiency

   7.2. Classification and nomenclature of enzymes

   7.3. Cofactors and coenzymes

     7.3.1. Cofactors

     7.3.2. Coenzymes

     7.3.3. Multi-substrate reactions

7.4.Mechanism of enzyme action

     7.4.1. Energy changes during chemical reactions

     7.4.2. Stages of enzymatic catalysis

     7.4.3. Molecular mechanisms of enzymatic catalysis

   7.5. Fundamentals of enzyme kinetics

     7.5.1. Dependence of enzymatic reaction rate on enzyme concentration

     7.5.2. Dependence of enzymatic reaction rate on ambient temperature

     7.5.3. Effect of medium pH on the enzymatic reaction rate

     7.5.4. Effect of substrate concentration on the enzymatic reaction rate

   7.6. Inhibition of enzymatic activity

     7.6.1. Reversible inhibition

     7.6.2. Irreversible inhibition

   7.7. Regulation of metabolic processes

     7.7.1. Organization of chemical reactions into metabolic pathways

     7.7.2. Principles of metabolic pathway regulation

     7.7.3. Main mechanisms for regulating enzyme catalytic activity

   7.8. Enzymopathies

   7.9. Application of enzymes in medicine

     7.9.1. Enzyme diagnostics

     7.9.2. Use of enzymes as therapeutic agents

   Review questions

Chapter 8. VITAMINS

8.1. Classification and general properties of vitamins

     8.1.1. Classification of vitamins

     8.1.2. History of the discovery of vitamins

8.2. Fat-soluble vitamins

     8.2.1. General biosynthetic pathways of fat-soluble vitamins

     8.2.2. Vitamin A group

     8.2.3. Vitamin D group

     8.2.4. Vitamin E group

     8.2.5. Vitamin K group

8.3. Water-soluble vitamins

     8.3.1. Vitamin B1 — Thiamine

     8.3.2. Vitamin B2 — Riboflavin

     8.3.3. Folic acid

     8.3.4. Vitamin PP — Niacin, nicotinic acid, nicotinamide

     8.3.5. Vitamin B5 — Pantothenic acid

     8.3.6. Vitamin B6 — Pyridoxine

     8.3.7. Vitamin B12 — Cobalamin

     8.3.8. Vitamin C

     8.3.9. Vitamin H — Biotin

     8.3.10. Vitamin-like compounds

   Review questions

Chapter 9. NUCLEIC ACIDS

   9.1. General characteristics of nucleic acids

   9.2. Components of nucleic acids

   9.3. Physicochemical properties of nitrogenous bases

9.4. Structure of nucleic acids

     9.4.1. Primary structure of nucleic acids

     9.4.2. Spatial organization of DNA structure

     9.4.3. Mitochondrial DNA

     9.4.4. DNA denaturation and renaturation

9.5. Structure and functions of ribonucleic acids

     9.5.1. Chemical nature of ribonucleic acids

     9.5.2. Biological functions of RNA

     9.5.3. Structural organization of RNA

   Review questions

Chapter 10. NUCLEIC ACID METABOLISM

   10.1. Nucleases

   10.2. Digestion of Nucleic Acids in the Gastrointestinal Tract

   10.3 Catabolism of Pyrimidine Bases

   10.4. Catabolism of Purine Bases

   10.5. Biosynthesis of Nucleotides

     10.5.1. Biosynthesis of Purine Nucleotides

     10.5.2 Biosynthesis of Pyrimidine Nucleotides

     10.5.3. Biological Functions of RNA

   10.6. DNA Biosynthesis (Replication)

     10.6.1. Replication Enzymes

     10.6.2 Mechanism of DNA Replication

     10.6.3. DNA Modification

   10.7. RNA Biosynthesis (Transcription)

     10.7.1. Transcription Enzymes

     10.7.2 Mechanism of DNA Transcription

     10.7.3. Processing of RNA Transcripts

   Review Questions

Chapter 11. STRUCTURE AND PROPERTIES OF CARBOHYDRATES

   11.1. Monosaccharides

   11.2. Oligosaccharides

   11.3 Polysaccharides

   Review Questions

Chapter 12. CARBOHYDRATE METABOLISM

12.1. Catabolic Pathways of Carbohydrates

     12.1. Carbohydrate Digestion

     12.2. Regulation of Blood Glucose Levels

     12.3 Glycolysis

     12.4. Pentose Phosphate Pathway of Carbohydrate Oxidation

     12.5. Phosphoketolase Pathway

   12.2 Gluconeogenesis

   Review Questions

Chapter 13. STRUCTURE AND PROPERTIES OF LIPIDS

   13.1. Structural Components of Lipids

   13.2. Neutral Lipids

   13.3. Phospholipids

   13.4. Sphingolipids

   Review Questions

Chapter 14. LIPID METABOLISM

   14.1. Lipid Transformations in the Gastrointestinal Tract

   14.2. Plasma Lipoproteins

   14.3. Degradation of Higher Fatty Acids

   14.4. Biosynthesis of Higher Fatty Acids

   14.5. Synthesis and Breakdown of Fats in Tissues

   14.6. Phosphoacylglycerol Metabolism

   14.7. Sphingolipid Metabolism

   14.8. Cholesterol Synthesis

   14.9. Ketone Body Metabolism

   Review Questions

Chapter 15. INTEGRATION OF METABOLIC PATHWAYS. HORMONES

   15.1. Compartmentation of metabolic pathways

   15.2. Classification of hormones

   15.3. Mechanisms of endocrine system function

   15.4. Mechanisms of hormone action

     15.4.1. Mechanisms of action of peptide hormones and catecholamines

     15.4.2. Mechanism of action of steroid and thyroid hormones

   15.5. Hypothalamic hormones

   15.6. Hormones of the adenohypophysis

   15.7. Vasopressin and oxytocin

   15.8. Hypothalamic hormones

   15.9. Corticosteroids

   15.10. Sex hormones

     15.10.1. Synthesis of male sex hormones

     15.10.2. Synthesis of female sex hormones

   15.11. Catecholamines

   15.12. Pancreatic hormones

   15.13. Parathyroid hormone and calcitonin

   15.14. Eicosanoids

   Review questions

Chapter 16. ENERGY METABOLISM

   16.1. Biological oxidation

     16.1.1. Characteristics of high-energy phosphates. ATP — ADP cycle

     16.1.2. Oxidation-reduction reactions. Redox potential

     16.1.3. Enzymes and coenzymes involved in oxidation-reduction reactions

     16.1.4. Organization of the mitochondrial respiratory chain

   16.2. Oxidative Phosphorylation of ADP

     16.2.1. Mechanism of Coupling between Oxidation and Phosphorylation

     16.2.2. Transport of ATP and ADP across Mitochondrial Membranes

     16.2.3. Uncoupling of Respiration and Phosphorylation

     16.2.4. Thermoregulatory Function of the Electron Transport Chain

   16.3. Final Stage of Catabolism: The Primary Source of Hydrogen Donors for the ETC

     16.3.1. Oxidative Decarboxylation of Pyruvate

     16.3.2. Hypoenergetic States

   16.4. Tricarboxylic Acid Cycle

   16.5. Generation of Reactive Oxygen Species in the ETC

   Review Questions

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