BIOCHEMISTRY - Textbook - Ostapchenko L. I. - 2012

Chapter 5. STRUCTURE, PROPERTIES, AND FUNCTIONS OF PROTEINS

5.7. Physicochemical properties of proteins and methods for their isolation

The isolation of individual Proteins from Organs and Tissues holds a prominent place in biochemical research. Purified individual Proteins are essential for studying their Primary Structure, obtaining protein crystals for Spatial Structure analysis via X-ray crystallography, and establishing the correlation between a protein's primary and spatial structures and its biological function.

Certain purified individual proteins are employed in medicine as therapeutic agents (for instance, the hormone Insulin is used to treat Diabetes Mellitus, while pancreatic digestive Enzymes are prescribed as enzyme replacement therapy for pancreatic dysfunction). Furthermore, purified enzymes are frequently utilized in biochemical research as chemical Reagents for quantifying substances in biological fluids.

Most Methods employed for the purification of individual proteins rely on differences in their physicochemical properties, as well as their capacity for specific Ligand binding.



Last update: 06/08/2026

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