Human Biochemistry Volume 2 - Murray R. 1993

Special Topics
Nutrition, Digestion, and Absorption
Carbohydrate Absorption

Carbohydrate Digestion products are absorbed from the jejunum into the Blood of the portal Venous system in the form of Monosaccharides, predominantly hexoses (glucose, fructose, mannose, and galactose) and pentoses. Oligosaccharides (compounds derived from starch that yield 3–10 monosaccharide units upon Hydrolysis) and Disaccharides are hydrolyzed by specific Enzymes located on the mucosal surface of the Small Intestine, which may include pancreatic amylase adsorbed onto the mucosa. The activity of free disaccharidases within the intestinal lumen is low, with the majority of their activity associated with small microvillar protrusions (the brush border) of the intestinal epithelial Cells.

The absorption of monosaccharides occurs via two primary mechanisms: Active Transport against a concentration gradient, and simple diffusion. However, the absorption of certain sugars cannot be strictly attributed to either mechanism alone. The molecular configuration features apparently required for active transport, which are characteristic of glucose and galactose, are as follows: the hydroxyl group at the C-2 position must have the same configuration as in glucose; a pyranose ring must be present; and a methyl or substituted methyl group must be located at the C-5 position. Fructose is absorbed more slowly than glucose and galactose, a process that appears to proceed via concentration-gradient-driven diffusion.

Class="center">Table 53.11. Summary of Digestive Processes

Source of Secretion and Stimulus

Enzyme

Activation Mechanism and Optimal Conditions

Substrate

End Product of Action

Salivary Glands: reflex secretion of saliva in response to food in the Oral Cavity

Salivary amylase

Requires chloride ion, pH 6.6–6.8

Starch

Glycogen

Maltose plus 1:6 glucosides (oligosaccharides) plus maltotriose

Lingual glands

Lingual lipase

pH range 2.0–7.5; optimum 4.0–4.5

Short-chain primary esters, sn-3 bond

Fatty acids plus 1,2-diacylglycerols

Gastric glands: chief and parietal cells secrete gastric juice in response to reflex stimulation and gastrin

Pepsin A (fundus)

Pepsin B (pylorus)

Pepsinogen is converted to active pepsin by HCl, pH 1.0–2.0

Protein

Peptides


Rennin

Requires calcium for activity, pH 4.0

Milk casein

Curdled milk

Pancreas:

entry of acidic chyme from The Stomach triggers the duodenum to produce 1) secretin, a hormone that stimulates pancreatic juice secretion; 2) cholecystokinin, which stimulates enzyme secretion

Trypsin

Trypsinogen is converted to active trypsin by intestinal enterokinase at pH 5.2–6.0. Autocatalytic conversion occurs at pH 7.9

Protein

Peptides

Polypeptides

Dipeptides


Chymotrypsin

Secreted as chymotrypsinogen and activated by trypsin, pH 8.0

Protein

Peptides

Same as for trypsin. Higher milk-clotting activity


Elastase

Secreted as proelastase and converted to its active form by trypsin

Protein

Peptides

Polypeptides

Dipeptides


Carboxypeptidase

Secreted as procarboxypeptidase, activated by trypsin

Polypeptides with a free C-terminal end

Smaller peptides, free Amino Acids


Pancreatic amylase

pH 7.1

Starch

Glycogen

Maltose plus 1:6 glucosides (oligosaccharides) plus maltotriose


Lipase

Activated by Bile salts, Phospholipids, colipase, pH 8.0

Primary ester bonds of triacylglycerols

Fatty acids, monoacylglycerols, diacylglycerols, glycerol


Ribonuclease


Ribonucleic acid

NUCLEOTIDES


Deoxyribonuclease


Deoxyribonucleic acid

Nucleotides


Cholesterol ester hydrolase

Activated by bile salts

Cholesterol esters

Free cholesterol plus fatty acids


Phospholipase A2

Secreted as a proenzyme, activated by trypsin and Ca2+

Phospholipids

Fatty acids, lysophospholipids

Liver and Gallbladder: cholecystokinin (an intestinal mucosal hormone) and, potentially, gastrin and secretin stimulate the gallbladder and hepatic bile secretion

(Bile salts and alkalis)


Fats; also neutralize acidic chyme

Fatty acid–bile salt conjugates, finely emulsified micelles of neutral fat and bile salts, and Liposomes

Small intestine: secretions of Brunner's glands in the duodenum and Lieberkühn's crypts

Aminopeptidase


Polypeptides with a free N-terminal end

Smaller peptides, free amino acids


Dipeptidases


Dipeptides

Amino acids


Sucrase

pH 5.0–7.0

Sucrose

Fructose, glucose


Maltase

pH 5.8–6.2

Maltose

Glucose


Lactase

pH 5.4–6.0

Lactose

Glucose, galactose


Trehalase


Trehalose

Glucose


Phosphatase

pH 8.6

Organic phosphates

Free phosphate


Isomaltase or 1:6 glucosidase


1:6 glucosides

Glucose


Polynucleotidase


Nucleic Acids

Nucleotides


Nucleosidases (nucleoside phosphorylases)


Purine or pyrimidine nucleosides

Purine or pyrimidine bases, pentose phosphate



Last update: 06/08/2026

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