FUNDAMENTALS OF ENZYME STRUCTURE AND KINETICS IN BIOLOGICAL SYSTEMS - O. A. Naumenko - 2017

Introduction

Studying the METABOLISM/9.html">Structure of Enzymes, their active sites, and the REGULATION OF ENZYMATIC reaction rates is a vital objective in biochemistry. Modern biotechnological and pharmaceutical industries widely employ enzyme Proteins as catalysts for synthesizing novel products and Pharmaceuticals; consequently, mastering the basics of enzymology is an essential milestone in the training of biologists.

The sections of this textbook are structured in accordance with the SYLLABUS OF THE module "Fundamentals of Enzyme Structure and Kinetics in Biological Systems" and comprise six chapters covering Enzyme Structure, the kinetics and Thermodynamics of enzymatic reactions, bioenergetics, a laboratory practicum, examination questions, and test Assignments. Each chapter includes Review Questions for self-Assessment of the acquired knowledge.

The laboratory exercises provided in the manual will enable students to develop skills in determining enzymatic reaction rates, calculating thermodynamic parameters under specified conditions, isolating proteins for research, mastering Protein Purification techniques via centrifugation and filtration, and assessing enzyme activity.

Class="center">Designations and Abbreviations

АДФ - adenosine diphosphate

АКО - amino acid residue

АМФ - adenosine monophosphate

АТФ - adenosine triphosphate

АлЦ - allosteric site

АЦ - Active Site

БАПНА - N-benzoyl-L-Arginine p-nitroanilide

НАД - nicotinamide adenine dinucleotide, oxidized

ВМП - inner mitochondrial membrane

ГФА - Glycogen transferase A

ГФВ - glycogen transferase B

ДНК - deoxyribonucleic acid

ФАД - flavin adenine dinucleotide, oxidized

ФМН - flavin mononucleotide

ХФР - chemical enzymatic reaction

ЦПЭ - Electron Transport Chain

ЦТК - Tricarboxylic Acid Cycle

цАМФ - cyclic AMP

НАДН - nicotinamide adenine dinucleotide, reduced

FADH2 - reduced flavin adenine dinucleotide

IMS - mitochondrial intermembrane space

RNA - ribonucleic acid

TS - thermodynamic system

TP - thermodynamic process



Last update: 06/08/2026

Editorial and Educational Adaptation: This material has been compiled based on the primary/original source text. The project team performed an editorial review, corrected technical inaccuracies, structured sections, and adapted the content for an educational format.

What was processed:

  • elimination of formatting defects (OCR errors, structural breaks, corrupted characters);
  • editorial organization of content;
  • standardization of terminology in accordance with academic sources;
  • verification of factual statements against the original source text.

All mentions of the author, publication year, and origin of the primary text have been preserved in accordance with the source.