PRACTICAL WORKBOOK IN HISTOLOGY, CYTOLOGY AND EMBRYOLOGY - 2016

Chapter 4. TISSUES

Slide 34. Holocrine sebaceous glands in hair-bearing skin.

Staining: hematoxylin and eosin.

Magnification: x80.

The holocrine secretion type is characterized by the glandular Cell filling with secretion, dying, and being discharged onto the epithelial surface. This is the mode of secretion in Sebaceous Glands of the Skin associated with Hair follicles. In the slide, the sebaceous gland appears as a cluster of large, pale Cells with vacuolated Cytoplasm (1). We can distinguish individual glandular acini (2) and branched excretory ducts (3) that open into the hair infundibulum (4). At the periphery of the acinus, There is a layer of small germinative cells (5) with large, spherical nuclei. As they approach the excretory duct, the cells acquire a polygonal shape, becoming sebocytes. Lipid inclusions accumulate in their cytoplasm. During Processing, the Lipids dissolve, resulting in The formation of rounded vacuoles within the cytoplasm. The nuclei shrink (karyopyknosis) and disintegrate (karyolysis). Eventually, The Cell membrane ruptures, and the group of cells transforms into a continuous mass of sebum that enters the excretory duct.

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Slide 34. Holocrine sebaceous glands in hair-bearing skin.



Last update: 07/08/2026

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