Practical Protein Chemistry - A. Darbre 1989

Enantiomeric analysis of amino acid mixtures by high-performance liquid chromatography
HPLC

A mixture (approx. 2 mg each) of the racemic Amino Acids (Table 9.1) is dissolved in 10.0 mL of Water. Cysteine and cystine are replaced in the mixture by cysteic acid (Cya) alone; Proline and hydroxyproline are absent.

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FIG. 9.1. Separation of acidic and neutral amino acids into three groups on a cation-exchange Column using 0.2 M pyridine-acetate buffer, pH 3.4. After 45 min, Trp, Lys, His, and Arg are eluted with 0.4 M buffer, pH 5.3. Temperature 50 °C; flow rate 0.17 mL/min [9]. (Reproduced by permission of Academic Press.)



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