Practical Protein Chemistry - A. Darbre 1989

Enantiomeric analysis of amino acid mixtures by high-performance liquid chromatography
Reagents
Synthesis of N, N-di-n-propyl-L-alanine

Commercially available Reagents are used for the analysis; Solvents are distilled, and Water is purified by double distillation. N,N-Di-n-propyl-L-Alanine is synthesized according to a slightly modified Procedure [1].

A suspension of 0.2 mol (17.8 g) of L-alanine (Sigma) in 200 mL of ethanol is prepared. Then, 3 g of catalyst—10% palladium on activated carbon (Finka)—and 43 mL of propionaldehyde (Finka) are added. The mixture is hydrogenated for 48 h at 30–40 °C under an initial hydrogen pressure of ~3.5 atm. The catalyst is removed using a Glass filter, and the filtrate is evaporated to dryness. The reaction product, N,N-di-n-propyl-L-alanine, is recrystallized from chloroform. The purity of the reagent is monitored by Thin-Layer Chromatography, nuclear magnetic Resonance, and elemental analysis.



Last update: 06/08/2026

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