Practical Protein Chemistry - A. Darbre 1989

Methods for Solid-Phase Amino Acid Sequence Analysis
Cleavage in a Solid-Phase Sequenator
Solvents

To wash the Column after interaction with FITC, methanol and 1,2-dichloroethane are usually used, the latter of which can be replaced by benzene. One of the recently proposed Microscale analysis protocols uses only methanol [52]. Higher yields are obtained using 0.2% propylamine in methanol. In this case, the buffer is added in portions, with nitrogen purging of the column in between (Fig. 12.5).



Last update: 06/08/2026

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