Practical Protein Chemistry - A. Darbre 1989

Separation of protein and peptide mixtures by high-performance liquid chromatography
Reversed-phase separation of peptides
Isolation of peptides using phosphoric acid

HPLC was used to monitor the Enzymatic Cleavage of the Calcitonin-binding protein [15]. The hydrolyzate was applied to the Column without prior Treatment. Peptide fractionation was performed in 0.1% phosphoric acid (pH 2.2) using an acetonitrile concentration gradient. During the preparative Isolation of the Peptides, the optical absorbance of the eluate was monitored at 210, 254, and 280 nm. The phosphoric acid remaining after fraction lyophilization did not interfere with Amino acid analysis, the determination of N-terminal Amino Acids using dansyl chloride, or automated amino acid sequencing. The enzymatic hydrolyzate was separated without preliminary treatment.



Last update: 06/08/2026

Editorial and Educational Adaptation: This material has been compiled based on the primary/original source text. The project team performed an editorial review, corrected technical inaccuracies, structured sections, and adapted the content for an educational format.

What was processed:

  • elimination of formatting defects (OCR errors, structural breaks, corrupted characters);
  • editorial organization of content;
  • standardization of terminology in accordance with academic sources;
  • verification of factual statements against the original source text.

All mentions of the author, publication year, and origin of the primary text have been preserved in accordance with the source.