Molecular Biology. A Practical Guide - Velikov V.A. 2013
Isolation of Total DNA from Cells
Isolation of DNA from Bacteria
Isolation of DNA from Blood Leukocytes
Isolation of DNA from Plant Tissues
DNA Concentration by Alcohol Precipitation
Spectrophotometry of DNA, RNA, and protein preparations
Spectrophotometric determination of DNA concentration
Spectrophotometry of DNA, RNA, and Protein Preparations
Determination of Protein Concentration via Intrinsic Fluorescence
Nucleic Acid Electrophoresis
Isolation of Plasmid and Phage DNA
Mini-preparation of Plasmid DNA (Miniprep)
Large-Scale Isolation of Plasmid DNA (Maxiprep)
Isolation of the M13 Phage Replicative Form (RF)
Isolation of M13 Single-Stranded Phage DNA
Restriction of Plasmid and Phage DNA
Restriction of Eukaryotic Genomic DNA
Performing DNA PCR amplification
Primer design and PCR parameter calculation using specialized software
Preparation of Vector DNA and Target Gene
DNA Ligation of Vector and Gene
Gene cloning
Restriction mapping of a DNA insert in a plasmid
Bacterial Transformation
Preparation of Competent E. coli Cells
Preparation of Electrocompetent E. coli Cells
Isolation of Recombinant Protein
Induced Expression of Cloned Genes
Extraction of Protein from the Periplasm of E. coli Cells
Isolation of recombinant protein
Dialysis of the protein preparation
Determination of Protein Concentration
Protein Concentration by TCA Precipitation
Protein Electrophoresis
Preparation of Solutions and Casting of PAGE
Performing Protein Electrophoresis
Coomassie R-250 Protein Staining
Calculations in Protein Work
Translation of Nucleotide Sequences
Calculations in Protein Research
Prediction of Nucleotide Sequence from Amino Acid Sequence