Molecular Biology. Practical Guide - Velikov V.A. 2013
Calculations in Protein Research
Analysis of Protein Amino Acid Sequences
A specialized program available on the MOLBIOL.RU website will help analyze a protein sequence: calculate its length, empirical formula, molecular weight, isoelectric point, and other parameters. To calculate the Molecular Weight of a labeled protein, the required isotopes must be checked. The «Help» page provides information on the margin of error and the calculation algorithm. Appendix 17 provides some of the most general reference data on Proteins.
1. Open the main page of the MOLBIOL.RU website (http://www.molbiol.ru).
2. On the main page, find the «Calculations» section.
3. Select the «Protein Sequence Analysis» tab.
4. Enter the protein sequence of the common viper NADH dehydrogenase into the form (see Practical Work 6.1), select the necessary options, and click «Calculate». The empirical formula and protein length are calculated regardless of the selected options.
5. Analyze a few more suggested proteins.
Last update: 13/08/2026
Editorial and Educational Adaptation: This material has been compiled based on the primary/original source text. The project team performed an editorial review, corrected technical inaccuracies, structured sections, and adapted the content for an educational format.
What was processed:
- elimination of formatting defects (OCR errors, structural breaks, corrupted characters);
- editorial organization of content;
- standardization of terminology in accordance with academic sources;
- verification of factual statements against the original source text.
All mentions of the author, publication year, and origin of the primary text have been preserved in accordance with the source.