PRACTICAL WORKBOOK ON HISTOLOGY, CYTOLOGY AND EMBRYOLOGY - 2016
Chapter 4. TISSUES
Slide 44. Reticular tissue (stroma of a lymph node).
Staining: hematoxylin and eosin.
Magnification: x400.
Under low magnification, locate an area of the Lymph node with the fewest lymphocytes, as the latter tend to obscure the reticular stroma. It is best to select clear spaces (medullary sinuses) in the central part of the section, and then study and sketch the identified area under high magnification.
The Cytoplasm of reticular Cells (1) is lightly stained, surrounds rounded nuclei, and forms processes through which these cells interconnect. In some areas, macrophages (2) and lymphocytes (3) can be observed. Trabeculae of Fibrous Connective Tissue and Blood vessel profiles are also occasionally visible.
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Slide 44. Reticular tissue (stroma of a lymph node).
Last update: 07/08/2026
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