BIOCHEMISTRY - L. Stryer - 1984

VOLUME 3

Part IV INFORMATION

CHAPTER 25. RNA INFORMATION AND TRANSCRIPTION

QUESTIONS AND PROBLEMS

1. Compare DNA polymerase I and E. coli RNA polymerase regarding each of the following properties:

а) Subunit Structure.

б) Activated precursors.

в) Direction of chain elongation.

г) Nuclease activities.

д) Template conservation.

е) Primer requirement.

ж) Energetics of the elongation reaction.

2. Write The sequence of the mRNA molecule synthesized on a DNA template strand with the following sequence:

5'-ATCGTACCGTTA-3'.

3. RNA is readily hydrolyzed by alkali, whereas DNA is not. Why?

4. How does cordycepin (3'-deoxyadenosine) block RNA Synthesis?

5. What products are expected from the partial Hydrolysis of the oligoribonucleotide 5'-pGCAGUACUGUC-3' by the following Enzymes:

а) pancreatic Ribonuclease,

б) ribonuclease T1,

в) ribonuclease U2,

г) ribonuclease I from Physarum?

6. Exhaustive hydrolysis of an oligoribonucleotide by pancreatic ribonuclease yields the following products: Cp, 2Up, AGCp, and GAAUp. Hydrolysis of the same oligoribonucleotide by ribonuclease T1 yields AAUp, UAGp, and CCUGp. What is its sequence?



Last update: 06/08/2026

Editorial and Educational Adaptation: This material has been compiled based on the primary/original source text. The project team performed an editorial review, corrected technical inaccuracies, structured sections, and adapted the content for an educational format.

What was processed:

  • elimination of formatting defects (OCR errors, structural breaks, corrupted characters);
  • editorial organization of content;
  • standardization of terminology in accordance with academic sources;
  • verification of factual statements against the original source text.

All mentions of the author, publication year, and origin of the primary text have been preserved in accordance with the source.