Protein Chemistry. Structure, Properties, and Research Methods - Shendryk A.N. 2022

Peptide Synthesis
Methods of Peptide Synthesis
Mixed Anhydride Method - Acid Chloride Method

This method employs acid chlorides of N-protected Amino Acids. The Procedure involves the following steps.

1. The N-protected amino acid chloride is obtained by treating The amino acid with a large excess of thionyl chloride in an inert solvent (such as benzene) or without a solvent, or via reaction with phosphorus pentachloride in benzene or chloroform:

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2. The peptide bond formation reaction is carried out with the amino component (NH2-a/k) in the presence of a strong base to bind the resulting hydrogen chloride:

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Last update: 06/08/2026

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