Practical Protein Chemistry - A. Darbre 1989

Determination of Amino Acid Sequence Using 4-Dimethylaminoazobenzene-4'-isothiocyanate (Manual Procedure)
Conclusion

Below are the main advantages of the DABITC—FITC method compared to standard manual amino acid sequencing Procedures.

1. Sensitivity: 1—10 nmol of peptide or protein is sufficient to determine a sequence of 20—30 residues, which is 10—20 times the capacity of other Methods.

2. Efficiency: a sequence of 20—30 Amino Acids can be established without major difficulties in both liquid-phase and solid-phase degradation modes. Thus, this method is significantly competitive with automated sequencing techniques.

3. Simplicity: implementing the Procedure requires only basic equipment. The Use of TLC, although it does not provide quantitative estimation, nevertheless allows all DABTH-Amino Acid Derivatives to be identified with a high degree of reliability.



Last update: 06/08/2026

Editorial and Educational Adaptation: This material has been compiled based on the primary/original source text. The project team performed an editorial review, corrected technical inaccuracies, structured sections, and adapted the content for an educational format.

What was processed:

  • elimination of formatting defects (OCR errors, structural breaks, corrupted characters);
  • editorial organization of content;
  • standardization of terminology in accordance with academic sources;
  • verification of factual statements against the original source text.

All mentions of the author, publication year, and origin of the primary text have been preserved in accordance with the source.