Practical Protein Chemistry - A. Darbre 1989

Amino acid sequence determination using 4-dimethylaminoazobenzene-4'-isothiocyanate (manual procedure)
Identification of DABTC amino acids
High-Performance Liquid Chromatography

DABTC Amino Acid Derivatives can be analyzed by reversed-phase HPLC (Fig. 14.4; conditions are given in the caption). The method allows for Quantitative evaluation of the results and is particularly convenient in combination with solid-phase degradation. However, the analysis does not reveal the color differences between the thiocarbamyl derivatives (blue) and the thiohydantoins (red) that are clearly visible on the thin-layer chromatogram.



Last update: 06/08/2026

Editorial and Educational Adaptation: This material has been compiled based on the primary/original source text. The project team performed an editorial review, corrected technical inaccuracies, structured sections, and adapted the content for an educational format.

What was processed:

  • elimination of formatting defects (OCR errors, structural breaks, corrupted characters);
  • editorial organization of content;
  • standardization of terminology in accordance with academic sources;
  • verification of factual statements against the original source text.

All mentions of the author, publication year, and origin of the primary text have been preserved in accordance with the source.