Practical Protein Chemistry - A. Darbre 1989

Disulfide Bonds
Fractionation of Cystine-Containing Peptides

The Isolation and Purification of cystine-containing Peptides represent a distinct challenge. The primary difficulties here are associated with the instability of Disulfide Bonds and the necessity of analyzing the resulting peptides using colorimetric Methods. In a weakly acidic medium (pH 2.0–6.5), disulfide bonds are relatively stable. This circumstance limits the choice of methods for polypeptide chain Cleavage and peptide isolation. Therefore, at the preliminary stage, it is necessary to determine The Structure of the half-cystine-containing fragments. Knowing the Primary Structure makes it much easier to design a cleavage Scheme for the native protein, allowing one to focus exclusively on the peptides containing disulfide bonds.



Last update: 06/08/2026

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