Practical Protein Chemistry - A. Darbre 1989

Application of electron impact mass spectrometry for determining the amino acid sequence of peptides and proteins
Interpretation of Mass Spectra
Introduction

The unambiguous Determination of the Amino Acid Sequence of a peptide by decoding a complex mass spectrum requires considerable experience, which is gained only through prolonged preliminary Study of the spectra of A large number of Peptides of known Structure. This section attempts to outline the principles of mass spectrum interpretation and, in this context, briefly describes the Main Pathways of peptide fragmentation under electron impact. A more detailed Discussion of peptide sequencing by mass spectrometry can be found in several previously published works [1, 3, 6–10, 24, 27, 28].



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