Practical Protein Chemistry - A. Darbre 1989

Determination of amino acid sequence using 4-dimethylaminoazobenzene-4'-isothiocyanate (manual procedure)
Conversion of amino acid thiazolinones to thiohydantoins

The extract containing The amino acid thiazolinone, obtained via liquid-phase or solid-phase degradation, is evaporated, and the residue is treated with a 5 M HCl–CH3COOH mixture (1:2) at 52 °C for 50 min. The sample is dried and redissolved in an appropriate volume of ethanol for subsequent identification.



Last update: 06/08/2026

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