Practical Protein Chemistry - A. Darbre 1989

Analytical Methods
Carbohydrate-Containing Proteins
Gas Chromatography-Mass Spectrometry Method

Intact Glycoproteins are methylated, hydrolyzed, reduced, and acetylated. Following Separation by Column Chromatography, the resulting sugar derivatives are analyzed by mass spectrometry. See review [250] on the application of GC, HPLC, and mass spectrometry in the analysis of carbohydrate complexes.



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