Practical Protein Chemistry - A. Darbre 1989

Affinity Chromatography of Proteins
Methods for Preparing Affinity Sorbents
Space Groups

Direct Condensation of a Ligand with the support can lead to steric constraints due to the relative inaccessibility of the active sites in the Proteins under study, thereby significantly reducing the efficiency of Affinity Chromatography. The Introduction of an intermediate spacer arm increases the distance between the support and the ligand, ensuring higher ligand accessibility. The decisive factors in determining the chemical nature and size of the spacer are The properties of both the ligand and the support.



Last update: 06/08/2026

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