ENVIRONMENTAL MICROBIOLOGY - M. I. Chernyavskaya - 2016

APPENDIX 2. MEDIA AND REAGENTS

Class="center">Peptone-Yeast Broth (PYB)

Enzymatic peptone 10.0 g

Yeast extract  5.0 g

NaCl   8.0 g

Distilled Water to 1 L

pH   7.0 — 7.2

Sterilize by autoclaving at 1.0 — 1.5 atm.

Peptone-Yeast Agar (PYA)

Enzymatic peptone 10.0 g

Yeast extract  5.0 g

NaCl   8.0 g

Microbiological agar 15.0 g

Distilled water to 1 L

pH   7.0 — 7.2

Sterilize by autoclaving at 1.0 — 1.5 atm.

Ashby's Medium

Mannitol   20.0 g

K2HPO4   0.2 g

MgSO4   0.2 g

NaCl   0.2 g

K2SO4   0.1 g

CaCO3   5.0 g

Microbiological agar 20.0 g

Distilled water up to 1 L

pH   7.1 — 7.3

Autoclave at 1.0 atm.

Casein-glycerol agar (CGA)

Casein hydrolysate  0.3 g

Yeast extract  0.3 g

Glycerol   10.0 mL

KNO3   2.0 g

К2НРO4   2.0 g

MgSO4 • 2 Н2O  0.05 g

FeSO4 • 7 Н2O  0.01 g

СаСO3   0.02 g

NaCl   2.0 g

Microbiological agar 20.0 g

Distilled water up to 1 L

pH   7.0 — 7.2

Autoclave at 1.0 — 1.5 atm.

Czapek's medium

Soluble starch 30.0 g

NaNO3   3.0 g

KH2PO4   1.0 g

MgSO4 • 7 Н2О  0.5 g

KCl   0.5 g

FeSO4 • 7 H2O  0.01 g

Microbiological agar 15.0 g

Distilled water to 1 L

pH   7.0 — 7.3

Sterilize by autoclaving at 1.5 atm.

Endo Agar

Peptone   10.0 g

Lactose   10.0 g

K2HPO4   3.5 g

Na2SO3   2.5 g

Basic fuchsin  0.5 g

Microbiological agar 15.0 g

Distilled water to 1 L

pH   7.3 — 7.5

Sterilize by autoclaving at 1.0 atm.

M9 Minimal Medium

M9 salt concentrate 25.0 mL

Distilled water 73.0 mL

0.1 M MgSO4  1.0 mL

0.1 M CaCl2  1.0 mL

pH   7.0 — 7.2

M9 Salt Concentrate

Na2HPO4   24.0 g

KH2PO4   12.0 g

NaCl   2.0 g

NH4Cl   4.0 g

Distilled water to 1 L

pH   7.0 — 7.2

Sterilize by autoclaving at 1.0 — 1.5 atm.

0.1 M MgSO4

MgSO4 (MgSO4 • 7 H2O) 1.20 g (2.46 g)

Distilled water to 1 L

Sterilize by boiling or autoclaving at 0.5 atm. 0.1

0.1 M CaCl2

CaCl2 (CaCl2 • 2 H2O)  1.11 g (1.47 g)

Distilled water to 1 L

Sterilize by boiling or autoclaving at 0.5 atm.

Physiological saline solution

NaCl   8.5 g

Distilled water to 1 L

pH   7.2 — 7.5

Sterilize by autoclaving at 1.5 atm.



Last update: 12/08/2026

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