MEDICAL BIOLOGY, HUMAN ANATOMY, PHYSIOLOGY AND PATHOLOGY - Y.I. Fedoniuk 2010

ANATOMY, PHYSIOLOGY, PATHOLOGY

SECTION 4. BLOOD SYSTEM

PROTECTIVE FUNCTIONS OF BLOOD

2. METHODOLOGY FOR LEUKOCYTE COUNTING

Dilute the Blood 20-fold with a 3% acetic acid solution containing gentian violet. This can be done in two ways: first, draw blood into a leukocyte pipette (Fig. 4.5) up to the 0.5 mark, and then draw in the acetic acid solution up to the 10.1 mark; second, add 0.4 ml of the gentian violet-stained 3% acetic acid solution into a clean, dry test tube, and then add 0.02 ml of blood using a micropipette.

Class="center">

Fig. 4.5. Leukocyte mixing pipette

Place a drop of the diluted blood onto a Goryaev chamber prepared in the same manner as for erythrocyte counting. Count the leukocytes under low magnification in 100 large squares grouped in sets of 4, using a darkened field of view.



Last update: 08/08/2026

Editorial and Educational Adaptation: This material has been compiled based on the primary/original source text. The project team performed an editorial review, corrected technical inaccuracies, structured sections, and adapted the content for an educational format.

What was processed:

  • elimination of formatting defects (OCR errors, structural breaks, corrupted characters);
  • editorial organization of content;
  • standardization of terminology in accordance with academic sources;
  • verification of factual statements against the original source text.

All mentions of the author, publication year, and origin of the primary text have been preserved in accordance with the source.