WORKSHOP ON HISTOLOGY, CYTOLOGY AND EMBRYOLOGY - 2016

Chapter 5. SPECIAL HISTOLOGY

Organs of Hematopoiesis and Immune Defense

Slide 84. Spleen.

Staining: hematoxylin and eosin.

Magnification: x400.

On one side of the section surface, we can observe a Connective Tissue capsule (1) covered by Peritoneum with mesothelium (2), from which trabeculae (3) extend deep into the organ. Red and white pulp are located between the trabeculae.

The white pulp consists of rounded lymphoid nodules (follicles) with a high density of purple nuclei belonging to lymphocytes (4). The nodule features a light (reactive) center (5) and a darker peripheral zone (mantle and marginal zone) (6). Lateral to the center, a central arteriole (7) is typically located, surrounded by an accumulation of T-lymphocytes, known as the periarteriolar zone. The accumulations of lymphocytes forming sheaths along the course of Arteries are called periarteriolar lymphoid sheaths. The red pulp (8) is composed of formed Blood elements, predominantly erythrocytes, located between the white pulp nodules. Trabecular arteries (9) and Veins are situated within the trabeculae (3).

Class="center">Cytology/practical/practical.files/image092.jpg" width="360"/>

J Slide 84. Spleen.



Last update: 07/08/2026

Editorial and Educational Adaptation: This material has been compiled based on the primary/original source text. The project team performed an editorial review, corrected technical inaccuracies, structured sections, and adapted the content for an educational format.

What was processed:

  • elimination of formatting defects (OCR errors, structural breaks, corrupted characters);
  • editorial organization of content;
  • standardization of terminology in accordance with academic sources;
  • verification of factual statements against the original source text.

All mentions of the author, publication year, and origin of the primary text have been preserved in accordance with the source.