Protein Structure and Function: Application of Bioinformatics Methods - John Rigden 2014

Bioinformatics Methods for Studying the Structure and Functions of Disordered Proteins
Disorder Prediction
Prediction Methods Based on the Absence of Well-Defined Secondary Structure

Another approach, although one without fundamental differences, relies on estimating the propensity of Amino Acids to form Secondary Structure elements (α-helices, β-strands, turns). It is based on the assumption that extended regions (exceeding 70 consecutive amino acid residues) lacking a predictable regular secondary structure are structurally disordered (Liu and Rost 2003). Performance metrics for the NORSp prediction method are comparable to those of other Methods. However, it should be noted that there are Proteins characterized by high orderliness yet consisting solely of non-repeating local structural elements (also referred to as loop proteins (Liu et al. 2002)), as well as IDPs containing transient local structural elements (Fuxreiter et al. 2004). The tendency of the latter to form ordered structures is readily predictable, which poses a fundamental limitation for predictions based on the principle described above.



Last update: 06/08/2026

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