Protein Chemistry. Structure, Properties, Research Methods - Shendryk A.N. 2022

Methods for Experimental Investigation of Protein Structure
Methods for Determining Protein Molecular Weight
Determination of Molecular Weight by Ebullioscopy and Cryoscopy

Raoult's law applied to ebullioscopy (cryoscopy) states that the elevation of the boiling point (or depression of the freezing point) of a solution relative to the pure solvent (ΔT) is directly proportional to the solution titer (s) and inversely proportional to the molar mass of the solute (M'):

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where k, k' are the ebullioscopic (cryoscopic) constants of the solvent.



Last update: 06/08/2026

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