Practical Protein Chemistry - A. Darbre 1989

Enzymatic fragmentation of the polypeptide chain
General conditions for enzymatic hydrolysis
Duration of enzymatic hydrolysis

According to the literature, complete Enzymatic Hydrolysis requires incubation with the enzyme for 2–4 hours. Reducing the incubation time to 10 minutes typically results in the Cleavage of only a few bonds most susceptible to the enzyme's action. For certain Enzymes or particularly stable substrates, the hydrolysis time is extended to 16–24 hours. In this case, a drop of toluene should be added to the reaction mixture to inhibit Microbial growth.



Last update: 06/08/2026

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