Practical Protein Chemistry - A. Darbre 1989

Peptide mapping of proteins
Conclusion

Currently, the most effective method for peptide Separation is reversed-phase HPLC. This method is characterized by experimental simplicity, speed, high sensitivity (especially for unlabeled samples), and excellent resolution. The separated components are in solution, which facilitates their subsequent analysis, such as determining Amino Acid Composition and sequence.



Last update: 06/08/2026

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