Practical Protein Chemistry - A. Darbre 1989

Disulfide Bonds
Identification and localization of cystine-containing peptides
Identification of disulfide bonds in proteins with unknown amino acid sequences

When analyzing a protein with an unknown Structure, one has to work "blindly." Thermolysin is typically used with great success to obtain cystine-containing Peptides that possess only a single disulfide bond [45]. This approach makes it possible to isolate and sequence peptides regardless of the arrangement order of half-cystine residues within the polypeptide chain. For comparative analysis, such as of structurally homologous Proteins, this method is quite acceptable. It yields sufficiently comprehensive information even before the Determination of the complete Primary Structure is finalized.



Last update: 06/08/2026

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